Mouse Talin ELISA Kit | TLN elisa kit

Mouse Talin ELISA Kit

Cat. Num. AAA761415

• Gene Names: TLN1; TLN; ILWEQ
• Reactivity: Mouse
• Synonyms: Talin; Mouse Talin ELISA Kit; TLN/KIAA1027/TLN1/ILWEQ/KIAA1027/talin 1/talin-1; TLN elisa kit

• Reactivity: Mouse
• Sequence Length: 2541

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TLN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse TLN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse TLN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TLN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse TLN can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6682361
• NCBI GeneID: 7094
• Molecular Weight: 269,767 Da
• NCBI Official Full Name: talin
• NCBI Official Synonym Full Names: talin 1
• NCBI Official Symbol: TLN1
• NCBI Official Synonym Symbols: TLN; ILWEQ
• NCBI Protein Information: talin-1
• UniProt Protein Name: Talin-1
• Protein Family: Talin
• UniProt Gene Name: TLN1
• UniProt Synonym Gene Names: KIAA1027; TLN
• UniProt Entry Name: TLN1_HUMAN

NCBI Description

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]

Uniprot Description

Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: cell surface; cytoplasm; cytosol; extracellular region; focal adhesion; intercellular junction; microtubule organizing center; plasma membrane; ruffle

Molecular Function: actin filament binding; integrin binding; LIM domain binding; protein binding; structural constituent of cytoskeleton; vinculin binding

Biological Process: axon guidance; blood coagulation; cell motility; cell-substrate junction assembly; cellular protein metabolic process; cortical actin cytoskeleton organization and biogenesis; cytoskeletal anchoring; intercellular junction assembly; muscle contraction; platelet activation; platelet degranulation; unfolded protein response; viral reproduction