Porcine Interleukin-18 binding prorein (IL-18BP) ELISA Kit | IL-18BP elisa kit

Porcine Interleukin-18 binding prorein (IL-18BP) ELISA Kit

Cat. Num. AAA261235

• Gene Names: IL18BP; IL18BPa
• Reactivity: Porcine
• Synonyms: Interleukin-18 binding prorein (IL-18BP); Porcine Interleukin-18 binding prorein (IL-18BP) ELISA Kit; IL-18BP elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 199

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for IL-18BP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine IL-18BP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 323098348
• NCBI GeneID: 10068
• NCBI Accession #: NP_005690.2
• NCBI GenBank Nucleotide #: NM_005699.3
• Molecular Weight: 17,478 Da
• NCBI Official Full Name: interleukin-18-binding protein isoform c
• NCBI Official Synonym Full Names: interleukin 18 binding protein
• NCBI Official Symbol: IL18BP
• NCBI Official Synonym Symbols: IL18BPa
• NCBI Protein Information: interleukin-18-binding protein; IL-18BP; tadekinig-alfa; MC51L-53L-54L homolog gene product
• UniProt Protein Name: Interleukin-18-binding protein
• UniProt Gene Name: IL18BP
• UniProt Synonym Gene Names: IL-18BP
• UniProt Entry Name: I18BP_HUMAN

NCBI Description

The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

IL18BP: Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; extracellular region

Molecular Function: receptor antagonist activity; interleukin-18 binding

Biological Process: T-helper 1 type immune response; response to lipopolysaccharide