Human Sex-determining region Y protein ELISA Kit | SRY elisa kit

Human Sex-determining region Y protein ELISA Kit

Cat. Num. AAA765000

• Gene Names: SRY; TDF; TDY; SRXX1; SRXY1
• Reactivity: Human
• Synonyms: Sex-determining region Y protein; Human Sex-determining region Y protein ELISA Kit; SRY/TDF/Sex-determining region Y protein/Testis-determining factor; SRY elisa kit

• Reactivity: Human
• Sequence Length: 204

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SRY elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human SRY antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SRY antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SRY amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SRY can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507225
• NCBI GeneID: 6736
• NCBI Accession #: NP_003131.1
• NCBI GenBank Nucleotide #: NM_003140.2
• Molecular Weight: 23,884 Da
• NCBI Official Full Name: sex-determining region Y protein
• NCBI Official Synonym Full Names: sex determining region Y
• NCBI Official Symbol: SRY
• NCBI Official Synonym Symbols: TDF; TDY; SRXX1; SRXY1
• NCBI Protein Information: sex-determining region Y protein
• UniProt Protein Name: Sex-determining region Y protein
• Protein Family: Sex-determining region Y protein
• UniProt Gene Name: SRY
• UniProt Synonym Gene Names: TDF
• UniProt Entry Name: SRY_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

SRY: Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. Involved in different aspects of gene regulation including promoter activation or repression. Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. Defects in SRY are the cause of 46,XY sex reversal type 1 (SRXY1). A condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Patients manifest rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. A 45,X chromosomal aberration involving SRY is found in Turner syndrome, a disease characterized by gonadal dysgenesis with short stature, streak gonads, variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female. Defects in SRY are the cause of 46,XX sex reversal type 1 (SRXX1). A condition in which male gonads develop in a genetic female (female to male sex reversal). Belongs to the SRY family.

Protein type: Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: Yp11.3

Cellular Component: cytoplasm; nuclear speck; nucleoplasm; nucleus

Molecular Function: calmodulin binding; DNA bending activity; DNA binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; transcription factor binding

Biological Process: cell differentiation; male gonad development; male sex determination; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent

Disease: 46,xx Sex Reversal 1; 46,xy Sex Reversal 1