Rabbit EKLF Polyclonal Antibody | anti-EKLF antibody

EKLF (W318) Polyclonal Antibody

Cat. Num. AAA3002443

• Gene Names: KLF1; EKLF; EKLF/KLF1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: EKLF; Polyclonal Antibody; EKLF (W318) Polyclonal Antibody; Krueppel-like factor 1; Erythroid krueppel-like transcription factor; KLF1; Krueppel-like factor 5; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; KLF5; BTEB2; CKLF; IKLF; Krueppel-like factor 7; Ubiquitous krueppel-like factor; KLF7; UKLF; Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor; KLF4; EZF; GKLF; anti-EKLF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EKLF (W318) polyclonal antibody detects endogenous levels of EKLF protein. This antibody also recognizes lKLF (51 kDa), GKLF (56 kDa), and UKLF (33 kDa).
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 362

• Applicable Applications for anti-EKLF antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 288-335 of Human EKLF.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of EKLF (W318) pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:SP2/0 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug))

Related Product Information for anti-EKLF antibody

The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Kruppel-like factors that have been characterized in mammals include EKLF, LKLF and GKLF. EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arrest.

NCBI and Uniprot Product Information

• NCBI GI #: 10835246
• NCBI GeneID: 10661
• NCBI Accession #: NP_006554.1
• NCBI GenBank Nucleotide #: NP_006554.1
• UniProt Accession #: Q13351
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: Krueppel-like factor 1
• NCBI Official Synonym Full Names: Kruppel like factor 1
• NCBI Official Symbol: KLF1
• NCBI Official Synonym Symbols: EKLF; EKLF/KLF1
• NCBI Protein Information: Krueppel-like factor 1
• UniProt Protein Name: Krueppel-like factor 1
• UniProt Gene Name: KLF1
• UniProt Synonym Gene Names: EKLF; EKLF

NCBI Description

This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009]

Uniprot Description

KLF1: Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4). It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.13

Cellular Component: nuclear chromatin; nucleus

Molecular Function: transcription factor activity

Biological Process: erythrocyte differentiation; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent

Disease: Anemia, Congenital Dyserythropoietic, Type Iv; Blood Group--lutheran Inhibitor; Fetal Hemoglobin Quantitative Trait Locus 6