Sheep Orosomucoid ELISA Kit | ORM elisa kit

Sheep Orosomucoid ELISA Kit

Cat. Num. AAA742938

• Gene Names: ORM1; ORM; AGP1; AGP-A; HEL-S-153w
• Reactivity: Sheep
• Synonyms: Orosomucoid; Sheep Orosomucoid ELISA Kit; ORM elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This ORM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ORM. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ORM elisa kit

Principle of the Assay: ORM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ORM antibody and an ORM-HRP conjugate. The assay sample and buffer are incubated together with ORM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ORM concentration since ORM from samples and ORM-HRP conjugate compete for the anti-ORM antibody binding site. Since the number of sites is limited, as more sites are occupied by ORM from the sample, fewer sites are left to bind ORM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ORM concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ORM elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 219519923
• NCBI GeneID: 5004
• Molecular Weight: 23,512 Da
• NCBI Official Full Name: Orosomucoid 1
• NCBI Official Synonym Full Names: orosomucoid 1
• NCBI Official Symbol: ORM1
• NCBI Official Synonym Symbols: ORM; AGP1; AGP-A; HEL-S-153w
• NCBI Protein Information: alpha-1-acid glycoprotein 1; AGP 1; OMD 1; orosomucoid-1; epididymis secretory sperm binding protein Li 153w
• UniProt Protein Name: Alpha-1-acid glycoprotein 1
• UniProt Gene Name: ORM1
• UniProt Synonym Gene Names: AGP1; AGP 1; OMD 1
• UniProt Entry Name: A1AG1_HUMAN

NCBI Description

This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. [provided by RefSeq, Jul 2008]

Uniprot Description

ORM1: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding

Biological Process: regulation of immune system process; transport; acute-phase response; inflammatory response