Western Blot (WB)
(Anti-Zonula occludens protein 3 antibody, MBS176134, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Heart Tissue Lysate )
Rabbit anti-Mouse, Rat Zonula occludens protein 3 Polyclonal Antibody | anti-TJP3 antibody
Anti-Zonula occludens protein 3 antibody
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Zonula occludens protein 3; Polyclonal Antibody; Anti-Zonula occludens protein 3 antibody; Tight junction protein ZO-3; tight junction protein 3; Tight junction protein 3 antibody; Tight junction protein ZO-3 antibody; Tight junction protein ZO3 antibody; TJP3 antibody; ZO3 antibody; ZO3_HUMAN antibody; Zona occludens protein 3 antibody; Zonula occludens protein 3 antibody; anti-TJP3 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
905
Applicable Applications for anti-TJP3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Zonula occludens protein 3 (885-905aa RARDVESSDDEGYDWGPATDL), identical to the related rat sequence.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Zonula occludens protein 3 antibody, MBS176134, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Heart Tissue Lysate )
Western Blot (WB)
(Anti-Zonula occludens protein 3 antibody, MBS176134, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Heart Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-Zonula occludens protein 3 antibody, MBS176134, IHC(P)IHC(P): Rat Intestine Tissue )
Immunohistochemistry (IHC)
(Anti-Zonula occludens protein 3 antibody, MBS176134, IHC(P)IHC(P): Rat Intestine Tissue )
Immunohistochemistry (IHC)
(Anti-Zonula occludens protein 3 antibody, MBS176134, IHC(P)IHC(P): Mouse Liver Tissue )
Immunohistochemistry (IHC)
(Anti-Zonula occludens protein 3 antibody, MBS176134, IHC(P)IHC(P): Mouse Liver Tissue )
Related Product Information for anti-TJP3 antibody
Description: Rabbit IgG polyclonal antibody for Tight junction protein ZO-3(TJP3) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: TJP3(TIGHT JUNCTION PROTEIN 3), also called ZO3, is a protein that in humans is encoded by the TJP3 gene. TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins(MAGUK) that associate with intracellular junctions(Itoh et al., 1999). Haskins et al.(1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds directly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al.(2002) determined that Tjp3 interacted with PATJ(INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.
Background: TJP3(TIGHT JUNCTION PROTEIN 3), also called ZO3, is a protein that in humans is encoded by the TJP3 gene. TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins(MAGUK) that associate with intracellular junctions(Itoh et al., 1999). Haskins et al.(1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds directly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al.(2002) determined that Tjp3 interacted with PATJ(INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
99,324 Da
NCBI Official Full Name
Tight junction protein ZO-3
NCBI Official Synonym Full Names
tight junction protein 3
NCBI Official Symbol
Tjp3
NCBI Protein Information
tight junction protein ZO-3; zona occludens protein 3; zonula occludens protein 3
UniProt Protein Name
Tight junction protein ZO-3
Protein Family
UniProt Gene Name
Tjp3
UniProt Synonym Gene Names
Zo3
UniProt Entry Name
ZO3_MOUSE
Uniprot Description
ZO3: Interacts with occludin, claudins and ZO-1. Interacts with INADL. Interacts with UBN1. Interacts with FASLG. Belongs to the MAGUK family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion
Cellular Component: nucleoplasm; cell surface; tight junction; membrane; apical plasma membrane; plasma membrane; cell junction; nucleus
Molecular Function: protein binding
Research Articles on TJP3
Similar Products
Product Notes
The TJP3 tjp3 (Catalog #AAA176134) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Zonula occludens protein 3 antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Zonula occludens protein 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the TJP3 tjp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zonula occludens protein 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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