Western Blot (WB)
(Western Blot analysis of ZNF828 expression in transfected 293T cell line by ZNF828 polyclonal antibody. Lane 1: C13orf8 transfected lysate (89.32kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human ZNF828 Polyclonal Antibody | anti-CHAMP1 antibody
ZNF828 (CHAMP1, C13orf8, CAMP, KIAA1802, ZNF828, Chromosome Alignment-maintaining Phosphoprotein 1, Zinc Finger Protein 828)
Gene Names
CHAMP1; CAMP; CHAMP; ZNF828; C13orf8
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ZNF828; Polyclonal Antibody; ZNF828 (CHAMP1; C13orf8; CAMP; KIAA1802; Chromosome Alignment-maintaining Phosphoprotein 1; Zinc Finger Protein 828); Anti -ZNF828 (CHAMP1; anti-CHAMP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human C13orf8.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MEAFQELRKPSARLECDHCSFRGTDYENVQIHMGTIHPEFCDEMDAGGLGKMIFYQKSAKLFHCHKCFFTSKMYSNVYYHITSKHASPDKWNDKPKNQLNKETDPVKSPPLPEHQKIPCNSAEPKSIPALSMETQKLGSVLSPESPKPTPLTPLEPQKPGSVVSPELQTPLPSPEPSKPASVSSPEPPKSVPVCESQKLAPVPSPEPQKPAPVSPESVKATLSNPKPQKQSHFPETLGPPSASSPESPVLAASPEPWGPSPAASPESRKSARTTSPEPRKPSPSESPEPWKPFPAVSPEPRRPAPAVSPGSWKPGPPGSPRPWKSNPSASSGPWKPAKPAPSVSPGPWKPIPSVSPGPWKPTPSVSSASWKSSSVSPSSWKSPPASPESWKSGPPELRKTAPTLSPEHWKAVPPVSPELRKPGPPLSPEIRSPAGSPELRKPSGSPDLWKLSPDQRKTSPASLDFPESQKSSRGGSPDLWKSSFFIEPQKPVFPETRKPGPSGPSESPKAASDIWKPVLSIDTEPRKPALFPEPAKTAPPASPEARKRALFPEPRKHALFPELPKSALFSESQKAVELGDELQIDAIDDQKCDILVQEELLASPKKLLEDTLFPSSKKLKKDNQESSDAELSSSEYIKTDLDAMDIKGQESSSDQEQVDVESIDFSKENKMDMTSPEQSRNVLQFTEEKEAFISEEEIAKYMKRGKGKYYCKICCCRAMKKGAVLHHLVNKHNVHSPYKCTICGKAFLLESLLKNHVAAHGQSLLKCPRCNFESNFPRGFKKHLTHCQSRHNEEANKKLMEALEPPLEEQQI
Applicable Applications for anti-CHAMP1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Full length human C13orf8, aa1-812 (NP_115812.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of ZNF828 expression in transfected 293T cell line by ZNF828 polyclonal antibody. Lane 1: C13orf8 transfected lysate (89.32kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ZNF828 expression in transfected 293T cell line by ZNF828 polyclonal antibody. Lane 1: C13orf8 transfected lysate (89.32kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to C13orf8 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to C13orf8 on HeLa cell. [antibody concentration 10ug/ml].)
Related Product Information for anti-CHAMP1 antibody
Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Product Categories/Family for anti-CHAMP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
89,099 Da
NCBI Official Full Name
ZNF828 protein
NCBI Official Synonym Full Names
chromosome alignment maintaining phosphoprotein 1
NCBI Official Symbol
CHAMP1
NCBI Official Synonym Symbols
CAMP; CHAMP; ZNF828; C13orf8
NCBI Protein Information
chromosome alignment-maintaining phosphoprotein 1; zinc finger protein 828
UniProt Protein Name
Chromosome alignment-maintaining phosphoprotein 1
Protein Family
UniProt Gene Name
CHAMP1
UniProt Synonym Gene Names
C13orf8; CAMP; KIAA1802; ZNF828
UniProt Entry Name
CHAP1_HUMAN
Uniprot Description
ZNF828: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Protein type: RNA-binding; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: nucleoplasm; cytoplasm; condensed chromosome; spindle; nucleus
Molecular Function: protein binding; nucleic acid binding; metal ion binding
Biological Process: sister chromatid biorientation; attachment of spindle microtubules to kinetochore during mitosis
Research Articles on CHAMP1
Similar Products
Product Notes
The CHAMP1 champ1 (Catalog #AAA6009034) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ZNF828 (CHAMP1, C13orf8, CAMP, KIAA1802, ZNF828, Chromosome Alignment-maintaining Phosphoprotein 1, Zinc Finger Protein 828) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF828 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the CHAMP1 champ1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MEAFQELRKP SARLECDHCS FRGTDYENVQ IHMGTIHPEF CDEMDAGGLG KMIFYQKSAK LFHCHKCFFT SKMYSNVYYH ITSKHASPDK WNDKPKNQLN KETDPVKSPP LPEHQKIPCN SAEPKSIPAL SMETQKLGSV LSPESPKPTP LTPLEPQKPG SVVSPELQTP LPSPEPSKPA SVSSPEPPKS VPVCESQKLA PVPSPEPQKP APVSPESVKA TLSNPKPQKQ SHFPETLGPP SASSPESPVL AASPEPWGPS PAASPESRKS ARTTSPEPRK PSPSESPEPW KPFPAVSPEP RRPAPAVSPG SWKPGPPGSP RPWKSNPSAS SGPWKPAKPA PSVSPGPWKP IPSVSPGPWK PTPSVSSASW KSSSVSPSSW KSPPASPESW KSGPPELRKT APTLSPEHWK AVPPVSPELR KPGPPLSPEI RSPAGSPELR KPSGSPDLWK LSPDQRKTSP ASLDFPESQK SSRGGSPDLW KSSFFIEPQK PVFPETRKPG PSGPSESPKA ASDIWKPVLS IDTEPRKPAL FPEPAKTAPP ASPEARKRAL FPEPRKHALF PELPKSALFS ESQKAVELGD ELQIDAIDDQ KCDILVQEEL LASPKKLLED TLFPSSKKLK KDNQESSDAE LSSSEYIKTD LDAMDIKGQE SSSDQEQVDV ESIDFSKENK MDMTSPEQSR NVLQFTEEKE AFISEEEIAK YMKRGKGKYY CKICCCRAMK KGAVLHHLVN KHNVHSPYKC TICGKAFLLE SLLKNHVAAH GQSLLKCPRC NFESNFPRGF KKHLTHCQSR HNEEANKKLM EALEPPLEEQ QI. It is sometimes possible for the material contained within the vial of "ZNF828, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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