Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using CHAMP1 antibody.)
Rabbit anti-Human, Mouse CHAMP1 Polyclonal Antibody | anti-CHAMP1 antibody
CHAMP1 Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CHAMP1; Polyclonal Antibody; CHAMP1 Polyclonal Antibody; CAMP; CHAMP; MRD40; ZNF828; C13orf8; anti-CHAMP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CHAMP1 polyclonal antibody detects endogenous levels of CHAMP1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
812
Applicable Applications for anti-CHAMP1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant protein of human CHAMP1
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using CHAMP1 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using CHAMP1 antibody.)
Related Product Information for anti-CHAMP1 antibody
Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 130kDa
NCBI Official Full Name
chromosome alignment-maintaining phosphoprotein 1
UniProt Protein Name
Chromosome alignment-maintaining phosphoprotein 1
Protein Family
UniProt Gene Name
CHAMP1
UniProt Synonym Gene Names
C13orf8; CAMP; CHAMP; KIAA1802; ZNF828
UniProt Entry Name
CHAP1_HUMAN
Uniprot Description
ZNF828: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Protein type: RNA-binding; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: nucleoplasm; cytoplasm; condensed chromosome; spindle; nucleus
Molecular Function: protein binding; nucleic acid binding; metal ion binding
Biological Process: sister chromatid biorientation; attachment of spindle microtubules to kinetochore during mitosis
Similar Products
Product Notes
The CHAMP1 champ1 (Catalog #AAA3009584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHAMP1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CHAMP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the CHAMP1 champ1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHAMP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
