Western Blot (WB)
(Western Blot (WB) analysis of Neuregulin-1 SMDF (Y4) pAb at 1:1000 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug))
Rabbit Neuregulin-1/SMDF Polyclonal Antibody | anti-SMDF antibody
Neuregulin-1/SMDF (Y4) Polyclonal Antibody
Gene Names
NRG1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Neuregulin-1/SMDF; Polyclonal Antibody; Neuregulin-1/SMDF (Y4) Polyclonal Antibody; Pro-neuregulin-1; membrane-bound isoform; Pro-NRG1; Neuregulin-1; Acetylcholine receptor-inducing activity; ARIA; Breast cancer cell differentiation factor p45; Glial growth factor; Heregulin; HRG; Neu differentiation factor; Sensory and motor neuron-derived factor; NRG1; GGF; HGL; HRGA; NDF; SMDF; anti-SMDF antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SMDF/Neuregulin 1 (Y4) polyclonal antibody detects endogenous levels of SMDF/Neuregulin 1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
459
Applicable Applications for anti-SMDF antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Neuregulin-1/SMDF.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Neuregulin-1 SMDF (Y4) pAb at 1:1000 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of Neuregulin-1 SMDF (Y4) pAb at 1:1000 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Neuregulin-1/SMDF (Y4) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Neuregulin-1/SMDF (Y4) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Related Product Information for anti-SMDF antibody
The neuregulins are a family of ErbB/HER ligands encoded by four genes. Neuregulin-1 gene, NRG1, encodes numerous splice variants with differing transcription initiation sites. Neuregulin-1 includes a range of isoforms with varying glycosylation, regulation of expression and function. Neuregulin-1 splice variants each bear an EGF-like domain, though otherwise have unique domain structures, differing functions and discrete tissue distribution. Six types of Neuregulin-1 isoform groups have been defined based on their structural features. Three types are most often described, type I (ARIA, NDF or HRG), type II (GGF) and type III (SMDF). Neuregulin-1 has been linked to schizophrenia and has diverse neural functions. Neuregulin-1 affects cell migration, the differentiation of neural crest and Schwann cells, and acts to upregulate the expression of acetylcholine receptors at muscle fibers during the formation of neuromuscular junctions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~ 44kDa
NCBI Official Full Name
pro-neuregulin-1, membrane-bound isoform isoform ndf43b
NCBI Official Synonym Full Names
neuregulin 1
NCBI Official Symbol
NRG1
NCBI Official Synonym Symbols
GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2
NCBI Protein Information
pro-neuregulin-1, membrane-bound isoform
UniProt Protein Name
Pro-neuregulin-1, membrane-bound isoform
UniProt Gene Name
NRG1
UniProt Synonym Gene Names
GGF; HGL; HRGA; NDF; SMDF; Pro-NRG1; ARIA; HRG
UniProt Entry Name
NRG1_HUMAN
NCBI Description
The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Apr 2016]
Research Articles on SMDF
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Product Notes
The SMDF nrg1 (Catalog #AAA3000600) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Neuregulin-1/SMDF (Y4) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neuregulin-1/SMDF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the SMDF nrg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuregulin-1/SMDF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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