Rabbit anti-Human ZNF148 Polyclonal Antibody | anti-ZNF148 antibody

ZNF148, NT (ZNF148, ZBP89, Zinc finger protein 148, Transcription factor ZBP-89, Zinc finger DNA-binding protein 89) (Biotin)

Cat. Num. AAA6365691

• Gene Names: ZNF148; BERF-1; BFCOL1; ZBP-89; ZFP148; pHZ-52; HT-BETA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZNF148; Polyclonal Antibody; NT (ZNF148; ZBP89; Zinc finger protein 148; Transcription factor ZBP-89; Zinc finger DNA-binding protein 89) (Biotin); Zinc finger DNA-binding protein 89; anti-ZNF148 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ZNF148 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ZNF148 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human ZNF148.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ZNF148 Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ZNF148 antibody detected the ZNF148 protein (arrow).)

Related Product Information for anti-ZNF148 antibody

ZNF148 is involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.

Product Categories/Family for anti-ZNF148 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Udelhoven, M., et al. J. Endocrinol. 204(2):199-208(2010). Chen, G.G., et al. Cancer Lett. 283(1):52-58(2009). Feng, Y., et al. FEBS J. 276(15):4197-4206(2009). Borghaei, R.C., et al. Biochem. Biophys. Res. Commun. 382(2):269-273(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 145386566
• NCBI GeneID: 7707
• NCBI Accession #: NP_068799.2
• NCBI GenBank Nucleotide #: NM_021964.2
• UniProt Accession #: Q9UQR1; O00389; O43591; Q58EY5; D3DN27
• Molecular Weight: 88,976 Da
• NCBI Official Full Name: zinc finger protein 148
• NCBI Official Synonym Full Names: zinc finger protein 148
• NCBI Official Symbol: ZNF148
• NCBI Official Synonym Symbols: BERF-1; BFCOL1; ZBP-89; ZFP148; pHZ-52; HT-BETA
• NCBI Protein Information: zinc finger protein 148; CACCC box-binding protein; transcription factor ZBP-89; CLL-associated antigen KW-10; zinc finger DNA-binding protein 89
• UniProt Protein Name: Zinc finger protein 148
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF148
• UniProt Synonym Gene Names: ZBP89
• UniProt Entry Name: ZN148_HUMAN

Uniprot Description

ZNF148: a transcription factor. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: nucleoplasm; Golgi apparatus

Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; substantia nigra development; gamete generation; cellular defense response; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent