Immunohistochemistry (IHC)
(mmunohistochemical staining (DAB) of paraffin embedded rat lung tissue using MBS612073 at a dilution of 1: 1000 incubated overnight. Secondary antibody was biotinylated goat anti-sheep (1: 3000) from ABC. This is a low power image showing intense staining of non-ciliated bronchiolar cells (Clara cells) and lesser staining in alveolar type II cells)
Rabbit anti-Rat Clathrin Polyclonal Antibody | anti-Chc antibody
Clathrin
Gene Names
Chc; CG9012; chc; CHC; Cla; CLH; D-Chc; dCHC; DmelCG9012; l(1)13Fb; l(1)G0438; l(1)VI
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry
Purity
SerumSerum
Synonyms
Clathrin; Polyclonal Antibody; Anti -Clathrin; anti-Chc antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
This antibody has been shown to be specific for clathrin. Species Crossreactivity: Rat, other species have not yet tested.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Applicable Applications for anti-Chc antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Immunogen
A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in mouse, human and bovine.
Cellular Localization
Cytoplasmic vesicle; cytoplasmic vesicle membrane; cytoplasmic side. Cell membrane; coated pit; cytoplasmic side. Melanosome. Note=Cytoplasmic face of coated pits and vesicles. PTM: The N-terminus is blocked. Belongs to the clathrin heavy chain family.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(mmunohistochemical staining (DAB) of paraffin embedded rat lung tissue using MBS612073 at a dilution of 1: 1000 incubated overnight. Secondary antibody was biotinylated goat anti-sheep (1: 3000) from ABC. This is a low power image showing intense staining of non-ciliated bronchiolar cells (Clara cells) and lesser staining in alveolar type II cells)
Immunohistochemistry (IHC)
(mmunohistochemical staining (DAB) of paraffin embedded rat lung tissue using MBS612073 at a dilution of 1: 1000 incubated overnight. Secondary antibody was biotinylated goat anti-sheep (1: 3000) from ABC. This is a low power image showing intense staining of non-ciliated bronchiolar cells (Clara cells) and lesser staining in alveolar type II cells)
Product Categories/Family for anti-Chc antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
clathrin heavy chain, isoform F
NCBI Official Synonym Full Names
Clathrin heavy chain
NCBI Official Symbol
Chc
NCBI Official Synonym Symbols
CG9012; chc; CHC; Cla; CLH; D-Chc; dCHC; DmelCG9012; l(1)13Fb; l(1)G0438; l(1)VI
NCBI Protein Information
CG9012 gene product from transcript CG9012-RE; CG9012-PA; CG9012-PB; CG9012-PC; CG9012-PD; CG9012-PE; CG9012-PF; Chc-PA; Chc-PB; Chc-PC; Chc-PD; Chc-PE; Chc-PF; clathrin; clathrin hc; clathrin heavy chain
Protein Family
Research Articles on Chc
Similar Products
Product Notes
The Chc (Catalog #AAA612073) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Clathrin reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Clathrin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Researchers should empirically determine the suitability of the Chc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Clathrin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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