Rabbit ZIPK, IN Polyclonal Antibody | anti-DAPK3 antibody

ZIPK, IN (ZIP Kinase)

Cat. Num. AAA613244

• Gene Names: DAPK3; ZIP; ZIPK; FLJ36473
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ZIPK; IN; Polyclonal Antibody; IN (ZIP Kinase); Anti -ZIPK; anti-DAPK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZIPK, IN (Zip Kinase). A 52kD band should be detected. Does not crossreact with DAP kinase. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-DAPK3 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot and Immunocytochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunocytochemistry: 10ug/ml
• Immunogen: Peptide corresponding to amino acids near the center of human ZIP Kinase.
• Positive Control: Jurkat or HeLa whole cell lysate.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of ZIP Kinase in Jurkat cells with MBS613244 at 10ug/ml.)

Western Blot (WB)

(Western Blot analysis of HeLa and Jurkat whole cell lysates with MBS613244.)

Testing Data

Related Product Information for anti-DAPK3 antibody

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family.

Product Categories/Family for anti-DAPK3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 116013581
• NCBI GeneID: 1613
• UniProt Accession #: Q05JY4; O43293
• Molecular Weight: 17,999 Da
• NCBI Official Full Name: ZIP kinase isoform
• NCBI Official Synonym Full Names: death-associated protein kinase 3
• NCBI Official Symbol: DAPK3
• NCBI Official Synonym Symbols: ZIP; ZIPK; FLJ36473
• NCBI Protein Information: death-associated protein kinase 3; dlk; ZIP-kinase; DAP kinase 3; DAP-like kinase; ZIP kinase isoform
• UniProt Protein Name: ZIP kinase isoform
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: ZIPK
• UniProt Entry Name: Q05JY4_HUMAN

NCBI Description

Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq]

Uniprot Description

DAPK3: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Monomer and homotrimer. Can also exist as homodimer or form heterodimers with ATF4. Homodimerization is required for activation segment autophosphorylation Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. According to PubMed:17953487, does not interact with PARW. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3. Interacts with AR and this interaction is enhanced by AATF. Interacts (via leucine zipper) with TCP10L (via leucine zipper). Interacts (via kinase domain) with DAPK1 (via kinase domain).Interacts with STAT3, NLK and TCF7L2. Isoform 1 and isoform 2 can interact with myosin and PPP1R12A. Isoform 2 is expressed in the bladder smooth muscle. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Tumor suppressor; Kinase, protein; Protein kinase, CAMK; CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: PML body; cytoplasm; nucleus; lipid raft

Molecular Function: protein serine/threonine kinase activity; identical protein binding; leucine zipper domain binding; protein binding; Rho GTPase binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; cAMP response element binding protein binding; ATP binding

Biological Process: regulation of smooth muscle contraction; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; protein amino acid autophosphorylation; regulation of myosin II filament assembly or disassembly; regulation of mitotic cell cycle; cytokinesis; chromatin modification; protein amino acid phosphorylation; neuron differentiation; regulation of apoptosis; regulation of cell shape; regulation of transcription, DNA-dependent; negative regulation of translation; regulation of focal adhesion formation; regulation of mitosis; regulation of autophagy; positive regulation of cell migration