Rabbit anti-Mouse DAPK3 / ZIP Kinase Polyclonal Antibody | anti-DAPK3 antibody

Rabbit Polyclonal to Mouse DAPK3 / ZIP Kinase

Cat. Num. AAA248601

• Gene Names: DAPK3; DLK; ZIP; ZIPK
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: DAPK3 / ZIP Kinase; Polyclonal Antibody; Rabbit Polyclonal to Mouse DAPK3 / ZIP Kinase; Anti-DAPK3 / ZIP Kinase Antibody (aa254-282) IHC-plus; DAPK3; DAP-like kinase; Dlk; MYPT1 kinase; ZIP kinase isoform; ZIP; DAP kinase 3; ZIP Kinase; ZIP-kinase; ZIPK; Mouse DAPK3; anti-DAPK3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Mouse DAPK3 / ZIP Kinase
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 454

• Applicable Applications for anti-DAPK3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (1:10 - 1:100), WB (1:1000)
• Target Species: Mouse
• Immunogen Description: KLH-conjugated synthetic peptide from internal region of Mouse Dapk3.
• Immunogen Type: Synthetic peptide
• Immunogen: DAPK3 / ZIP Kinase antibody was raised against kLH-conjugated synthetic peptide from internal region of Mouse Dapk3.
• Antigen Modification: aa254-282
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Mouse Dapk3 Antibody (Center) Western blot of mouse testis tissue lysates (35 ug/lane). This demonstrates the Dapk3 antibody detected the Dapk3 protein (arrow).)

Related Product Information for anti-DAPK3 antibody

DAPK3, a DAPK type protein kinase, is functions in apoptotic signaling and also is believed to function in coordination of specific transcription and splicing events. DAPK3 contains a leucine zipper motif at its C terminus in addition to the N terminal kinase domain. DAPK3 binds to ATF4, a member of the activating transcription factor/cyclic AMP-responsive element-binding protein (ATF/CREB) family.

Product Categories/Family for anti-DAPK3 antibody

Family: Protein Kinase; Subfamily: DAPK

NCBI and Uniprot Product Information

• NCBI GI #: 4557511
• NCBI GeneID: 1613
• NCBI Accession #: NP_001339.1
• NCBI GenBank Nucleotide #: NM_001348.2
• UniProt Accession #: O43293; Q05JY4; A0AVN4; B3KQE2
• Molecular Weight: 37,048 Da
• NCBI Official Full Name: death-associated protein kinase 3
• NCBI Official Synonym Full Names: death-associated protein kinase 3
• NCBI Official Symbol: DAPK3
• NCBI Official Synonym Symbols: DLK; ZIP; ZIPK
• NCBI Protein Information: death-associated protein kinase 3; ZIP-kinase; DAP kinase 3; MYPT1 kinase; DAP-like kinase; zipper-interacting protein kinase
• UniProt Protein Name: Death-associated protein kinase 3
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK3
• UniProt Synonym Gene Names: ZIPK; DAP kinase 3; Dlk; ZIP-kinase
• UniProt Entry Name: DAPK3_HUMAN

NCBI Description

Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

DAPK3: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Monomer and homotrimer. Can also exist as homodimer or form heterodimers with ATF4. Homodimerization is required for activation segment autophosphorylation Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. According to PubMed:17953487, does not interact with PARW. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3. Interacts with AR and this interaction is enhanced by AATF. Interacts (via leucine zipper) with TCP10L (via leucine zipper). Interacts (via kinase domain) with DAPK1 (via kinase domain).Interacts with STAT3, NLK and TCF7L2. Isoform 1 and isoform 2 can interact with myosin and PPP1R12A. Isoform 2 is expressed in the bladder smooth muscle. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: PML body; cytoplasm; nucleus; lipid raft

Molecular Function: protein serine/threonine kinase activity; identical protein binding; leucine zipper domain binding; protein binding; protein homodimerization activity; Rho GTPase binding; calmodulin-dependent protein kinase activity; cAMP response element binding protein binding; ATP binding

Biological Process: regulation of smooth muscle contraction; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; regulation of myosin II filament assembly or disassembly; regulation of mitotic cell cycle; cytokinesis; chromatin modification; protein amino acid phosphorylation; regulation of apoptosis; neuron differentiation; regulation of cell shape; regulation of transcription, DNA-dependent; negative regulation of translation; regulation of focal adhesion formation; regulation of mitosis; regulation of autophagy; positive regulation of cell migration