Rabbit anti-Human, Mouse ZIP5 Polyclonal Antibody | anti-SLC39A5 antibody

ZIP5 Antibody

Cat. Num. AAA151235

• Gene Names: SLC39A5; ZIP5; MYP24; LZT-Hs7
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: ZIP5 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ZIP5; Polyclonal Antibody; ZIP5 Antibody; LZT-Hs7; Zinc transporter ZIP5; Solute carrier family 39 member 5; ZIP-5; solute carrier family 39 (metal ion transporter); member 5; anti-SLC39A5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ZIP5 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; ZIP5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 540

• Applicable Applications for anti-SLC39A5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF)
• Application Notes: ZIP5 antibody can be used for detection of ZIP5 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL.; Optimal dilutions for each application to be determined by the researcher.; ; Antibody validated: Western Blot in human samples; Immunohistochemistry in humansamples and Immunofluorescence in human samples. All other applications and speciesnot yet tested.
• Conjugate: Unconjugated
• Immunogen: ZIP5 antibody was raised against a 17 amino acid synthetic peptide near the center of human ZIP5.; The immunogen is located within amino acids 330-380 of ZIP5
• Positive Control: 1) CAT No: MBS151374 - Human Spleen Tissue Lysate; 2) CAT No: MBS154333- Human Spleen Tissue Slide
• Preparation and Storage: ZIP5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ZIP5 in human spleen tissue lysate with ZIP5 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ZIP5 in human spleen tissue with ZIP5 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ZIP5 in human spleen tissue with ZIP5 antibody at 20 μg/mL.)

Related Product Information for anti-SLC39A5 antibody

ZIP5 Antibody: The zinc transporter ZIP5, also known as SLC39A5, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP5 is closely related to ZIP4, and is found in many of the same tissues, including intestine, pancreas, liver, and kidney, but unlike ZIP4, ZIP5 localizes to the basolateral membrane in kidney cells and its activity is not downregulated by zinc repletion. Recent evidence suggests that ZIP4 and ZIP5 are reciprocally regulated, with translation of ZIP5 mRNA decreasing and ZIP5 protein being rapidly internalized and degraded in response to zinc deficiency.

NCBI and Uniprot Product Information

• NCBI GI #: 206597541
• NCBI GeneID: 283375
• NCBI Accession #: NP_001128667
• NCBI GenBank Nucleotide #: NM_001135195.1
• UniProt Accession #: Q6ZMH5; Q8N6Y3; B2R808
• NCBI Official Full Name: zinc transporter ZIP5
• NCBI Official Synonym Full Names: solute carrier family 39 (zinc transporter), member 5
• NCBI Official Symbol: SLC39A5
• NCBI Official Synonym Symbols: ZIP5; MYP24; LZT-Hs7
• NCBI Protein Information: zinc transporter ZIP5; zrt- and Irt-like protein 5; solute carrier family 39 member 5; solute carrier family 39 (metal ion transporter), member 5
• UniProt Protein Name: Zinc transporter ZIP5
• Protein Family: Zinc transporter
• UniProt Gene Name: SLC39A5
• UniProt Synonym Gene Names: ZIP5; ZIP-5
• UniProt Entry Name: S39A5_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ZIP family of zinc transporters that transport zinc into cells from outside, and play a crucial role in controlling intracellular zinc levels. Zinc is an essential cofactor for many enzymes and proteins involved in gene transcription, growth, development and differentiation. Mutations in this gene have been associated with autosomal dominant high myopia (MYP24). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

SLC39A5: May play a role in polarized cells by carrying out serosal-to-mucosal zinc transport. Seems to play a central role in controlling organismal zinc status. Belongs to the ZIP transporter (TC 2.A.5) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: basolateral plasma membrane; integral to membrane

Molecular Function: metal ion transmembrane transporter activity

Biological Process: positive regulation of nuclear mRNA splicing, via spliceosome; BMP signaling pathway; eye development; zinc ion transport; transmembrane transport

Disease: Myopia 24, Autosomal Dominant