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Western Blot (WB)
(Western blot analysis of extracts from Hela, using ZFYVE19 Antibody. Lane 1 was treated with the blocking peptide.)
Rabbit anti-Human, Mouse ZFYVE19 Polyclonal Antibody | anti-ZFYVE19 antibody
ZFYVE19 Antibody
Gene Names
ZFYVE19; ANCHR; MPFYVE
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZFYVE19; Polyclonal Antibody; ZFYVE19 Antibody; Abscission/NoCut checkpoint regulator; ANCHR; MLL partner containing FYVE domain; MPFYVE; ZFY19_HUMAN; ZFYVE 19; Zfyve19; Zinc finger FYVE domain containing protein 19; Zinc finger FYVE domain-containing protein 19; Zinc finger FYVE type containing 19; Zinc finger; FYVE domain containing 19; anti-ZFYVE19 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ZFYVE19 antibody detects endogenous levels of total ZFYVE19
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
471
Applicable Applications for anti-ZFYVE19 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoskeleton
Tissue Specificity
Detected in brain, heart, skeletal muscle, kidney and liver.
Predicted Cross Reactivity
Horse, Rabbit, Dog
Similarity
Horse (87%), Rabbit (100%), Dog (86%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Hela, using ZFYVE19 Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hela, using ZFYVE19 Antibody. Lane 1 was treated with the blocking peptide.)
Immunohistochemistry (IHC)
(MBS9606079 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9606079 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells, using ZFYVE19 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells, using ZFYVE19 antibody.)
Immunofluorescence (IF)
(MBS9606079 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9606079 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-ZFYVE19 antibody
Function: Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.
Subunit Structure: Interacts (via MIM1-B) with VPS4A; interaction takes place at the midbody ring following cytokinesis checkpoint activation.
Similarity: The FYVE-type zinc finger mediates binding to phosphatidylinositol-3-phosphate (PtdIns3P).The MIM1-B motif mediates interaction with VPS4A.
Subunit Structure: Interacts (via MIM1-B) with VPS4A; interaction takes place at the midbody ring following cytokinesis checkpoint activation.
Similarity: The FYVE-type zinc finger mediates binding to phosphatidylinositol-3-phosphate (PtdIns3P).The MIM1-B motif mediates interaction with VPS4A.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 50 kD
Predicted: 52 kDa
Predicted: 52 kDa
NCBI Official Full Name
abscission/NoCut checkpoint regulator isoform 1
NCBI Official Synonym Full Names
zinc finger FYVE-type containing 19
NCBI Official Symbol
ZFYVE19
NCBI Official Synonym Symbols
ANCHR; MPFYVE
NCBI Protein Information
abscission/NoCut checkpoint regulator
UniProt Protein Name
Abscission/NoCut checkpoint regulator
Protein Family
UniProt Gene Name
ZFYVE19
UniProt Synonym Gene Names
ANCHR; MPFYVE; ANCHR
Uniprot Description
Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.
Research Articles on ZFYVE19
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Product Notes
The ZFYVE19 zfyve19 (Catalog #AAA9606079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZFYVE19 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZFYVE19 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ZFYVE19 zfyve19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZFYVE19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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