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Western Blot (WB) (Western Blot analysis in mouse Neuro-2a cell line lysates (15ug/lane) using MBS6006272. This demonstrates that MBS6006272 detected the ZDHC2 protein (arrow).)

Rabbit anti-Human, Mouse ZDHC2 Polyclonal Antibody

ZDHC2, NT (ZDHHC2, REAM, REC, ZNF372, Palmitoyltransferase ZDHHC2, Reduced expression associated with metastasis protein, Reduced expression in cancer protein, Zinc finger DHHC domain-containing protein 2, Zinc finger protein 372)

Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
ZDHC2; Polyclonal Antibody; NT (ZDHHC2; REAM; REC; ZNF372; Palmitoyltransferase ZDHHC2; Reduced expression associated with metastasis protein; Reduced expression in cancer protein; Zinc finger DHHC domain-containing protein 2; Zinc finger protein 372); Anti -ZDHC2; anti-ZDHC2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-ZDHC2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
ZDHC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-114 amino acids from the N-terminal region of human ZDHC2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse Neuro-2a cell line lysates (15ug/lane) using MBS6006272. This demonstrates that MBS6006272 detected the ZDHC2 protein (arrow).)

Western Blot (WB) (Western Blot analysis in mouse Neuro-2a cell line lysates (15ug/lane) using MBS6006272. This demonstrates that MBS6006272 detected the ZDHC2 protein (arrow).)

Western Blot (WB)

(Western Blot analysis of MBS6006272 pre-incubated without (lane 1) and with (lane 2) blocking peptide in Ramos cell line lysate.)

Western Blot (WB) (Western Blot analysis of MBS6006272 pre-incubated without (lane 1) and with (lane 2) blocking peptide in Ramos cell line lysate.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6006272 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006272 for immunohistochemistry.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6006272 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006272 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Neuro-2a cells using MBS6006272 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Neuro-2a cells using MBS6006272 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ZDHC2 antibody
Palmitoyltransferase specific for GAP43 and DLG4/PSD95 (By similarity).
Product Categories/Family for anti-ZDHC2 antibody

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Product Notes

The ZDHC2 (Catalog #AAA6006272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZDHC2, NT (ZDHHC2, REAM, REC, ZNF372, Palmitoyltransferase ZDHHC2, Reduced expression associated with metastasis protein, Reduced expression in cancer protein, Zinc finger DHHC domain-containing protein 2, Zinc finger protein 372) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZDHC2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the ZDHC2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZDHC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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