Rabbit anti-Human, Mouse ZDHC2 Polyclonal Antibody | anti-ZDHC2 antibody

ZDHC2, NT (ZDHHC2, REAM, REC, ZNF372, Palmitoyltransferase ZDHHC2, Reduced expression associated with metastasis protein, Reduced expression in cancer protein, Zinc finger DHHC domain-containing protein 2, Zinc finger protein 372) (MaxLight 490)

Cat. Num. AAA6364980

• Gene Names: ZDHHC2; DHHC2; ZNF372
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ZDHC2; Polyclonal Antibody; NT (ZDHHC2; REAM; REC; ZNF372; Palmitoyltransferase ZDHHC2; Reduced expression associated with metastasis protein; Reduced expression in cancer protein; Zinc finger DHHC domain-containing protein 2; Zinc finger protein 372) (MaxLight 490); ZDHHC2; Zinc finger protein 372; anti-ZDHC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZDHC2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-ZDHC2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 77-106 of human ZDHC2, UniProt Accession #Q9UIJ5.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse Neuro-2a cell line lysates (15ug/lane) usingMBS6006272. This demonstrates that MBS6006272 detected the ZDHC2 protein (arrow).)

Western Blot (WB)

(Western Blot analysis of MBS6006272 pre-incubated without (lane 1) and with (lane 2) blocking peptide in Ramos cell line lysate.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6006272 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006272 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Neuro-2a cells using MBS6006272 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ZDHC2 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009). 2. Sharma, C., et al. Mol. Biol. Cell 19(8):3415-3425(2008). 3. Zhang, J., et al. Mol. Cell Proteomics 7(7):1378-1388(2008). 4. Li, B., et al. J. Biol. Chem. 277(32):28870-28876(2002). 5. Oyama, T., et al. Genes Chromosomes Cancer 29(1):9-15(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 7705949
• NCBI GeneID: 51201
• NCBI Accession #: NP_057437.1
• NCBI GenBank Nucleotide #: NM_016353.4
• UniProt Accession #: Q9UIJ5; D3DSP5
• Molecular Weight: 42,022 Da
• NCBI Official Full Name: palmitoyltransferase ZDHHC2
• NCBI Official Synonym Full Names: zinc finger, DHHC-type containing 2
• NCBI Official Symbol: ZDHHC2
• NCBI Official Synonym Symbols: DHHC2; ZNF372
• NCBI Protein Information: palmitoyltransferase ZDHHC2; rec; ream; DHHC-2; zinc finger protein 372; reduced expression in cancer protein; zinc finger, DHHC domain containing 2; zinc finger DHHC domain-containing protein 2; reduced expression associated with metastasis protein
• UniProt Protein Name: Palmitoyltransferase ZDHHC2
• UniProt Gene Name: ZDHHC2
• UniProt Synonym Gene Names: REAM; REC; ZNF372; Ream; Rec; DHHC-2
• UniProt Entry Name: ZDHC2_HUMAN

Uniprot Description

ZDHHC2: Palmitoyltransferase specific for GAP43 and DLG4/PSD95. Mutations in ZDHHC2 are found in hepatocellular carcinoma and colorectal cancer. Belongs to the DHHC palmitoyltransferase family.

Protein type: EC 2.3.1.225; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: Golgi apparatus; integral to plasma membrane; endoplasmic reticulum; recycling endosome membrane

Molecular Function: zinc ion binding; palmitoyltransferase activity; protein-cysteine S-palmitoleyltransferase activity

Biological Process: protein palmitoylation