Rabbit Atg4D Polyclonal Antibody | anti-Atg4D antibody

Atg4D

Cat. Num. AAA191003

• Gene Names: ATG4D; APG4D; AUTL4; APG4-D
• Applications: Immunoblot, Western Blot
• Synonyms: Atg4D; Polyclonal Antibody; anti-Atg4D antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody recognizes human Atg4D.
• Form/Format: Peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 411

• Applicable Applications for anti-Atg4D antibody: Immunoblotting (IB)
• Application Notes: Immunoblotting: use at 1-2ug/ml. A band of ~52kDa is detected.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Synthetic peptide corresponding to amino acids at the C-terminus of human Atg4D.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Immunofluorescence (IF)

Testing Data

Related Product Information for anti-Atg4D antibody

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. The protein encoded by the Atg5 gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Two transcript variants encoding different protein isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 528078306
• NCBI GeneID: 84971
• NCBI Accession #: NP_001268433.1
• NCBI GenBank Nucleotide #: NM_001281504.1
• UniProt Accession #: Q86TL0; Q969K0
• Molecular Weight: 16,008 Da
• NCBI Official Full Name: cysteine protease ATG4D isoform 2
• NCBI Official Synonym Full Names: autophagy related 4D cysteine peptidase
• NCBI Official Symbol: ATG4D
• NCBI Official Synonym Symbols: APG4D; AUTL4; APG4-D
• NCBI Protein Information: cysteine protease ATG4D
• UniProt Protein Name: Cysteine protease ATG4D
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4D
• UniProt Synonym Gene Names: APG4D; AUTL4

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ATG4D: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Belongs to the peptidase C54 family.

Protein type: Autophagy; EC 3.4.22.-; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity

Biological Process: autophagic vacuole formation; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane