Rabbit anti-Human, Rat PLK3 Polyclonal Antibody | anti-PLK3 antibody

PLK3 Antibody

Cat. Num. AAA7045722

• Gene Names: PLK3; CNK; FNK; PRK; PLK-3
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: PLK3; Polyclonal Antibody; PLK3 Antibody; Serine/threonine-protein kinase PLK3; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3; Proliferation-related kinase; CNK; FNK; PRK; anti-PLK3 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 646

• Applicable Applications for anti-PLK3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human Serine/threonine-protein kinase PLK3 protein (C-160AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9580 / sc-9578 / sc-25422
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: PLK3 antibody at 1.2ug/mlLane 1: Rat heart tissueLane 2: Hela whole cell lysateLane 3: 293T whole cell lysateLane 4: MCF7 whole cell lysateLane 5: Monocytic leukemia cells in mice MacrophagesSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 72 kDaObserved band size: 72 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7045722 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7045722 at dilution 1:100)

Related Product Information for anti-PLK3 antibody

Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.

NCBI and Uniprot Product Information

• NCBI GI #: 41872374
• NCBI GeneID: 1263
• NCBI Accession #: NP_004064.2
• NCBI GenBank Nucleotide #: NM_004073.3
• UniProt Accession #: Q9H4B4; Q15767; Q5JR99; Q96CV1
• Molecular Weight: 71,629 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK3
• NCBI Official Synonym Full Names: polo like kinase 3
• NCBI Official Symbol: PLK3
• NCBI Official Synonym Symbols: CNK; FNK; PRK; PLK-3
• NCBI Protein Information: serine/threonine-protein kinase PLK3
• UniProt Protein Name: Serine/threonine-protein kinase PLK3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PLK3
• UniProt Synonym Gene Names: CNK; FNK; PRK; PLK-3
• UniProt Entry Name: PLK3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015]

Uniprot Description

PLK3: a kinase of the PLK family. Phosphorylated and activated following DNA damage or mitotic spindle disruption. Interacts with Chk2 and the interaction is enhanced upon DNA damage. Apparent substrates include Chk2 and p53. Plays an important role in regulating microtubule dynamics and centrosomal function. Deregulated expression of Plk3 results in cell cycle arrest and apoptosis.

Protein type: EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Nucleolus; Kinase, protein; Other group; PLK family

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: centrosome; cytoplasm; Golgi stack; nucleolus; nucleoplasm; nucleus

Molecular Function: p53 binding; protein binding; protein serine/threonine kinase activity

Biological Process: apoptosis; cytoplasmic microtubule organization and biogenesis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; endomitotic cell cycle; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; mitotic cell cycle checkpoint; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; protein kinase B signaling cascade; regulation of cell division; regulation of cytokinesis; response to DNA damage stimulus; response to osmotic stress; response to radiation; response to reactive oxygen species