Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-4: A172, Hela, 293T and 231 cell lysates, Primary antibody: 823592(MORC2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Rabbit anti-Human ZCWCC1 Polyclonal Antibody | anti-ZCWCC1 antibody
ZCWCC1 Rabbit pAb
Gene Names
MORC2; ZCW3; ZCWCC1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
ZCWCC1; Polyclonal Antibody; ZCWCC1 Rabbit pAb; MORC family CW-type zinc finger 2; ZCW3; anti-ZCWCC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Highly expressed in smooth muscle, pancreas and testis. Gene expression databases
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-ZCWCC1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC: 1:25-1:100
ELISA: 1:5000-1:10,000
IHC: 1:25-1:100
Immunogen
Fusion protein of human MORC2
Conjugation
Unconjugated
Molecular Function
Essential for epigenetic silencing by the HUSH (human silencing hub) complex. Recruited by HUSH to target site in heterochromatin, the ATPase activity and homodimerization are critical for HUSH-mediated silencing (PubMed:28581500, PubMed:29440755). Represses germ cell-related genes and L1 retrotransposons in collaboration with SETDB1 and the HUSH complex, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). During DNA damage response, regulates chromatin remodeling through ATP hydrolysis. Upon DNA damage, is phosphorylated by PAK1, both colocalize to chromatin and induce H2AFX expression. ATPase activity is required and dependent of phosphorylation by PAK1 and presence of DNA (PubMed:23260667). Recruits histone deacetylases, such as HDAC4, to promoter regions, causing local histone H3 deacetylation and transcriptional repression of genes such as CA9 (PubMed:20225202, PubMed:20110259). Exhibits a cytosolic function in lipogenesis, adipogenic differentiation, and lipid homeostasis by increasing the activity of ACLY, possibly preventing its dephosphorylation (PubMed:24286864).
Modification
Unmodified
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-4: A172, Hela, 293T and 231 cell lysates, Primary antibody: 823592(MORC2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-4: A172, Hela, 293T and 231 cell lysates, Primary antibody: 823592(MORC2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 823592(MORC2 Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 823592(MORC2 Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 823592(MORC2 Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 823592(MORC2 Antibody) at dilution 1/20. (Original magnification: ×200))
Related Product Information for anti-ZCWCC1 antibody
The CW domain is a structural module found in many vertebrate, parasitic and plant proteins. It consists of a mononuclear four-cysteine zinc-finger domain that plays a role in DNA binding, chromatin methylation and early embryonic development. ZCWCC1 (zinc finger CW-type coiled-coil domain protein 1), also known as MORC2 (MORC family CW-type zinc finger protein 2) or ZCW3, is a 1, 032 amino acid protein that contains one CW-type zinc finger domain. ZCWCC1 is located on chromosome 22 and is ubiquitously expressed with highest expression in pancreas, smooth muscle and testis. Expression of ZCWCC1 is upregulated in hypoxia, a pathological condition characterized by an inadequate supply of oxygen in the blood.
Product Categories/Family for anti-ZCWCC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
117,823 Da
NCBI Official Full Name
MORC family CW-type zinc finger protein 2
NCBI Official Synonym Full Names
MORC family CW-type zinc finger 2
NCBI Official Symbol
MORC2
NCBI Official Synonym Symbols
ZCW3; ZCWCC1
NCBI Protein Information
MORC family CW-type zinc finger protein 2; zinc finger, CW type with coiled-coil domain 1; zinc finger, CW-type with coiled-coil domain 1; zinc finger CW-type coiled-coil domain protein 1
UniProt Protein Name
MORC family CW-type zinc finger protein 2
UniProt Gene Name
MORC2
UniProt Synonym Gene Names
KIAA0852; ZCWCC1
UniProt Entry Name
MORC2_HUMAN
Similar Products
Product Notes
The ZCWCC1 morc2 (Catalog #AAA4758089) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZCWCC1 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZCWCC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC: 1:25-1:100. Researchers should empirically determine the suitability of the ZCWCC1 morc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZCWCC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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