Rabbit anti-Human ZBP1 Polyclonal Antibody | anti-ZBP1 antibody

ZBP1, CT (Insulin-like Growth Factor 2 mRNA-binding Protein 1, IGF2 mRNA-binding Protein 1, IMP-1, Coding Region Determinant-binding Protein, CRD-BP, IGF-II mRNA-binding Protein 1, VICKZ Family Member 1, Zip Code-binding Protein 1, ZBP-1,. Zipcode-binding

Cat. Num. AAA643120

• Gene Names: ZBP1; DAI; DLM1; DLM-1; C20orf183
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ZBP1; Polyclonal Antibody; CT (Insulin-like Growth Factor 2 mRNA-binding Protein 1; IGF2 mRNA-binding Protein 1; IMP-1; Coding Region Determinant-binding Protein; CRD-BP; IGF-II mRNA-binding Protein 1; VICKZ Family Member 1; Zip Code-binding Protein 1; ZBP-1; . Zipcode-binding; Anti -ZBP1; anti-ZBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human ZBP1. Species sequence homology: mouse and rat
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-ZBP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 18aa from near the C-terminal of human ZBP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Liver (formalin-fixed, paraffin-embedded) stained with ZBP1 antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Western blot analysis of ZBP1 in mouse small intestine tissue lysate with ZBP1 antibody at (A) 0.5, (B) 1 and (C) 2ug/ml.)

Related Product Information for anti-ZBP1 antibody

yZ-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Zalpha domain which binds specifically to left-handed Z-DNA and Z-RNA. ZBP1 was initially identified as a novel gene that was up-regulated in activated macrophages in mice bearing ascites tumors, suggesting that is may play a role in processes such as host response in neoplasia. More recent reports indicate that the cytosolic ZBP1 has can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of ZBP1 to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK). Multiple isoforms of ZBP1 are known to exist.

Product Categories/Family for anti-ZBP1 antibody

Antibodies; Abs to Nucleic Acids

NCBI and Uniprot Product Information

• NCBI GI #: 238550212
• NCBI GeneID: 81030
• NCBI Accession #: NP_001153891.1
• NCBI GenBank Nucleotide #: NM_001160419.2
• UniProt Accession #: Q9H171; Q5JY39; Q9BYW4; A2A2F7; B3KVA1; F5GYT1
• Molecular Weight: 46,343 Da
• NCBI Official Full Name: Z-DNA-binding protein 1 isoform d
• NCBI Official Synonym Full Names: Z-DNA binding protein 1
• NCBI Official Symbol: ZBP1
• NCBI Official Synonym Symbols: DAI; DLM1; DLM-1; C20orf183
• NCBI Protein Information: Z-DNA-binding protein 1; DNA-dependent activator of IRFs; DNA-dependent activator of IFN-regulatory factors; tumor stroma and activated macrophage protein DLM-1; DNA-dependent activator of interferon regulatory factors
• UniProt Protein Name: Z-DNA-binding protein 1
• Protein Family: Z-DNA-binding protein
• UniProt Gene Name: ZBP1
• UniProt Synonym Gene Names: C20orf183; DLM1
• UniProt Entry Name: ZBP1_HUMAN

NCBI Description

This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

ZBP1: Participates in the detection by the host's innate immune system of DNA from viral, bacterial or even host origin. Plays a role in host defense against tumors and pathogens. Acts as a cytoplasmic DNA sensor which, when activated, induces the recruitment of TBK1 and IRF3 to its C-terminal region and activates the downstream interferon regulatory factor (IRF) and NF-kappa B transcription factors, leading to type-I interferon production. ZBP1-induced NF-kappaB activation probably involves the recruitment of the RHIM containing kinases RIPK1 and RIPK3. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; left-handed Z-DNA binding; RNA binding

Biological Process: metabolic process; positive regulation of interferon type I production; innate immune response