Rabbit ZBP1 Polyclonal Antibody | anti-ZBP1 antibody

ZBP1 Antibody

Cat. Num. AAA9408450

• Gene Names: ZBP1; DAI; DLM1; DLM-1; C20orf183
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: ZBP1; Polyclonal Antibody; ZBP1 Antibody; Z-DNA binding protein 1; DLM-1; DAI; anti-ZBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 406

• Applicable Applications for anti-ZBP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 18 amino acid peptide from near the carboxy terminus of human ZBP1.
• Target Name: ZBP1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ZBP1 in mouse small intestine tissue lysate with ZBP1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ZBP1 in human small intestine tissue with ZBP1 antibody at 2.5 ug/mL.)

Related Product Information for anti-ZBP1 antibody

Z-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Zalpha domain which binds specifically to left-handed Z-DNA and Z-RNA. ZBP1 was initially identified as a novel gene that was up-regulated in activated macrophages in mice bearing ascites tumors, suggesting that is may play a role in processes such as host response in neoplasia. More recent reports indicate that the cytosolic ZBP1 has can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of ZBP1 to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK). Multiple isoforms of ZBP1 are known to exist.

Product Categories/Family for anti-ZBP1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 119595916
• NCBI GeneID: 81030
• Molecular Weight: 38,220 Da
• NCBI Official Full Name: Z-DNA binding protein 1, isoform CRA_b
• NCBI Official Synonym Full Names: Z-DNA binding protein 1
• NCBI Official Symbol: ZBP1
• NCBI Official Synonym Symbols: DAI; DLM1; DLM-1; C20orf183
• NCBI Protein Information: Z-DNA-binding protein 1
• UniProt Protein Name: Z-DNA-binding protein 1
• Protein Family: Z-DNA-binding protein
• UniProt Gene Name: ZBP1
• UniProt Synonym Gene Names: C20orf183; DLM1
• UniProt Entry Name: ZBP1_HUMAN

NCBI Description

This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

ZBP1: Participates in the detection by the host's innate immune system of DNA from viral, bacterial or even host origin. Plays a role in host defense against tumors and pathogens. Acts as a cytoplasmic DNA sensor which, when activated, induces the recruitment of TBK1 and IRF3 to its C-terminal region and activates the downstream interferon regulatory factor (IRF) and NF-kappa B transcription factors, leading to type-I interferon production. ZBP1-induced NF-kappaB activation probably involves the recruitment of the RHIM containing kinases RIPK1 and RIPK3. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; left-handed Z-DNA binding; RNA binding

Biological Process: metabolic process; positive regulation of interferon type I production; innate immune response