Typical Testing Data/Standard Curve (for reference only)
Human MT3 ELISA Kit | MT3 elisa kit
Human MT3 (Metallothionein 3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MT3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MT3. You can calculate the concentration of Human MT3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family.
Chromosomal Location of Human Ortholog: 16q13
Cellular Component: synaptic vesicle; perinuclear region of cytoplasm; cytoplasm; inclusion body; intracellular
Molecular Function: antioxidant activity; protein binding; copper ion binding; zinc ion binding; caspase inhibitor activity; drug binding; cadmium ion binding; protein kinase activator activity
Biological Process: positive regulation of catalytic activity; negative regulation of autophagy; removal of superoxide radicals; positive regulation of transcription, DNA-dependent; negative regulation of axon extension; negative regulation of caspase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; zinc ion homeostasis; cadmium ion homeostasis; cellular lipid catabolic process; histone modification; protein kinase B signaling cascade; negative regulation of neuron apoptosis; cholesterol catabolic process; regulation of response to food; protein stabilization; activation of protein kinase B; cellular zinc ion homeostasis; zinc ion transport; regulation of protein amino acid glycosylation; leptin-mediated signaling pathway; astrocyte development; cell proliferation; cellular metal ion homeostasis; protein import into nucleus, translocation; negative regulation of oxidoreductase activity; energy reserve metabolic process; response to hypoxia; negative regulation of cell growth; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Research Articles on MT3
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Product Notes
The Human MT3 mt3 (Catalog #AAA2501265) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2501265 ELISA Kit recognizes Human MT3. It is sometimes possible for the material contained within the vial of "MT3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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