Rabbit anti-Human ZAK Polyclonal Antibody | anti-ZAK antibody

ZAK (Phospho-S165) polyclonal antibody

Cat. Num. AAA3015069

• Gene Names: ZAK; pk; AZK; MLT; MRK; MLK7; MLTK; mlklak
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: ZAK; Polyclonal Antibody; ZAK (Phospho-S165) polyclonal antibody; Mitogen-activated protein kinase kinase kinase MLT; Human cervical cancer suppressor gene 4 protein; HCCS-4; Leucine zipper- and sterile alpha motif-containing kinase; MLK-like mitogen-activated protein triple kinase; Mixed lineage kinase-related kinase; MLK-related kinase; MRK; Sterile alpha motif- and leucine zipper-containing kinase AZK; MLTK; HCCS4; anti-ZAK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ZAK (Phospho-S165) polyclonal antibody detects endogenous levels of ZAK protein only when phosphorylated at Ser165.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ZAK antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic phosphopeptide derived from human ZAK around the phosphorylation site of Serine 165
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of ZAK (Phospho-S165) polyclonal antibody at 1:500 dilutionLaneA: SGC7901 whole cell lysateLaneB: HEK293T whole cell lysateLaneC: HCT116 whole cell lysate)

Related Product Information for anti-ZAK antibody

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

NCBI and Uniprot Product Information

• NCBI GI #: 82880648
• NCBI GeneID: 51776
• NCBI Accession #: NP_057737.2
• NCBI GenBank Nucleotide #: NM_016653.2
• UniProt Accession #: Q9NYL2; Q53SX1; Q580W8; Q59GY5; Q86YW8; Q9HCC4; Q9HCC5; Q9HDD2; Q9NYE9; B3KPG2
• Molecular Weight: 91,155 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase MLT isoform 1
• NCBI Official Symbol: ZAK
• NCBI Official Synonym Symbols: pk; AZK; MLT; MRK; MLK7; MLTK; mlklak
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase MLT; HCCS-4; mixed lineage kinase 7; mixed lineage kinase-related kinase MRK-beta; human cervical cancer suppressor gene 4 protein; MLK-like mitogen-activated protein triple kinase; mixed lineage kinase with
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase MLT
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MLTK
• UniProt Synonym Gene Names: ZAK; HCCS-4; MLK-related kinase; MRK
• UniProt Entry Name: MLTK_HUMAN

NCBI Description

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ZAK: a mixed-lineage kinase containing a leucine-zipper (LZ) and a sterile-alpha motif (SAM). It is reported to activate the JNK/SAPK and NF-kappaB pathways.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding

Biological Process: cell death; cell proliferation; response to radiation; activation of MAPKK activity; positive regulation of apoptosis; DNA damage checkpoint; response to stress; cytoskeleton organization and biogenesis; cell cycle arrest; cell differentiation; protein amino acid phosphorylation; cell cycle checkpoint; activation of JNK activity