Rabbit anti-Human MDM4 Polyclonal Antibody | anti-MDM4 antibody

MDM4, ID (MDM4, MDMX, Protein Mdm4, Protein Mdmx, Double minute 4 protein, Mdm2-like p53-binding protein, p53-binding protein Mdm4) (Azide free) (HRP)

Cat. Num. AAA6319841

• Gene Names: MDM4; HDMX; MDMX; MRP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MDM4; Polyclonal Antibody; ID (MDM4; MDMX; Protein Mdm4; Protein Mdmx; Double minute 4 protein; Mdm2-like p53-binding protein; p53-binding protein Mdm4) (Azide free) (HRP); p53-binding protein Mdm4; anti-MDM4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MDM4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MDM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-157 amino acids from the Central region of human MDM4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MDM4 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the MDM4 antibody detected the MDM4 protein (arrow).)

Related Product Information for anti-MDM4 antibody

The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene.

Product Categories/Family for anti-MDM4 antibody

Antibodies; Binding Proteins

References

Xu, N., et al. Biochem. Biophys. Res. Commun. 401(3):417-421(2010). Sarkari, F., et al. J. Mol. Biol. 402(5):825-837(2010). Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Fang, S., et al. PLoS ONE 5 (5), E10813 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 88702791
• NCBI GeneID: 4194
• NCBI Accession #: NP_002384.2
• NCBI GenBank Nucleotide #: NM_002393.4
• UniProt Accession #: O15151; Q2M2Y2; Q32SL2; Q6GS18; Q8IV83
• Molecular Weight: 49,541 Da
• NCBI Official Full Name: protein Mdm4 isoform 1
• NCBI Official Synonym Full Names: MDM4, p53 regulator
• NCBI Official Symbol: MDM4
• NCBI Official Synonym Symbols: HDMX; MDMX; MRP1
• NCBI Protein Information: protein Mdm4; protein Mdmx; MDM4 protein variant G; MDM4 protein variant Y; MDM4-related protein 1; mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog; double minute 4, human homolog of; p53-binding protein
• UniProt Protein Name: Protein Mdm4
• Protein Family: Protein
• UniProt Gene Name: MDM4
• UniProt Synonym Gene Names: MDMX
• UniProt Entry Name: MDM4_HUMAN

NCBI Description

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

MDM4: Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with UPB2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53. Down-regulated by cisplatin. Expressed in all tissues tested with high levels in thymus. Belongs to the MDM2/MDM4 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; zinc ion binding

Biological Process: cell proliferation; negative regulation of cell proliferation; protein stabilization; DNA damage response, signal transduction by p53 class mediator; G0 to G1 transition; positive regulation of cell proliferation; protein complex assembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein catabolic process; negative regulation of apoptosis