Rabbit anti-Human RHD Polyclonal Antibody | anti-RHD antibody

RHD, ID (RHD, Blood group Rh(D) polypeptide, RHXIII, Rh polypeptide 2, Rhesus D antigen, CD240D) (PE)

Cat. Num. AAA6341872

• Gene Names: RHD; RH; Rh4; RH30; RhII; RhPI; DIIIc; RHCED; RHDel; RHPII; RhDCw; CD240D; RHXIII; RHDVA(TT); RhK562-II
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibody prior to conjugation was purified by Protein A affinity chromatography followed by peptide affinity purification.
• Synonyms: RHD; Polyclonal Antibody; ID (RHD; Blood group Rh(D) polypeptide; RHXIII; Rh polypeptide 2; Rhesus D antigen; CD240D) (PE); CD240D; anti-RHD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RHD.
• Purity/Purification: Antibody prior to conjugation was purified by Protein A affinity chromatography followed by peptide affinity purification.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RHD antibody: Western Blot (WB)
• Application Notes: WB: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide derived from a portion of amino acids within the region aa173-201 amino acids in the central region of human RHD.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of A549 cell line lysates (35ug/lane) usingMBS643423.)

Product Categories/Family for anti-RHD antibody

Antibodies; CD Markers

References

Flegr, J., et al. Folia Parasitol. 57(2):143-150(2010). Liu, H.C., et al. Biochim. Biophys. Acta 1800(6):565-573(2010). Schmid, P., et al. Transfusion 50(1):267-269(2010). Wang, X.D., et al. Eur. J. Clin. Invest. 39(7):607-617(2009). Wagner, F.F., et al. Blood 99(6):2272-2273(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 189095266
• NCBI GeneID: 6007
• NCBI Accession #: NP_001121163.1; NP_057208.2
• NCBI GenBank Nucleotide #: NM_001127691.2
• UniProt Accession #: Q02161; Q02162; Q07618; Q16147; Q16235; Q16355; Q5VSK0; Q5XLS9; Q5XLT1; Q5XLT2; Q9NPK0; Q9UQ20
• Molecular Weight: 41,694 Da
• NCBI Official Full Name: blood group Rh(D) polypeptide isoform 2
• NCBI Official Synonym Full Names: Rh blood group D antigen
• NCBI Official Symbol: RHD
• NCBI Official Synonym Symbols: RH; Rh4; RH30; RhII; RhPI; DIIIc; RHCED; RHDel; RHPII; RhDCw; CD240D; RHXIII; RHDVA(TT); RhK562-II
• NCBI Protein Information: blood group Rh(D) polypeptide
• UniProt Protein Name: Blood group Rh(D) polypeptide
• UniProt Gene Name: RHD
• UniProt Synonym Gene Names: RhPII

NCBI Description

The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.