Rabbit anti-Human XEDAR Polyclonal Antibody | anti-EDA2R antibody

XEDAR Antibody

Cat. Num. AAA151283

• Gene Names: EDA2R; XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: XEDAR Antibody is affinity chromatography purified via peptide column.
• Synonyms: XEDAR; Polyclonal Antibody; XEDAR Antibody; EDAA2R; EDA-A2R; TNFRSF27; UNQ2448/PRO5727/PRO34080; Tumor necrosis factor receptor superfamily member 27; X-linked ectodysplasin-A2 receptor; EDA-A2 receptor; ectodysplasin A2 receptor; anti-EDA2R antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: XEDAR Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 297

• Applicable Applications for anti-EDA2R antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: XEDAR antibody can be used for the detection of XEDAR by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: XEDAR antibody was raised against recombinant human XEDAR.
• Buffer: XEDAR Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: XEDAR antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of XEDAR in 293 cell lysate with XEDAR antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of XEDAR in human skin tissue with XEDAR antibody at 10 μg/mL.)

Related Product Information for anti-EDA2R antibody

XEDAR Antibody: X-linked ectodysplasin-A2 receptor (XEDAR) is a recently isolated member of the tumor necrosis factor receptor family that is highly expressed during embryonic development and binds to ectodysplatin-A2 (EDA-A2). Two predominantly expressed isoforms, XEDAR-s and XEDAR-L, differ by only a 21-amino region at the juxtamembrane region of the cytoplasmic domain. Neither isoform possesses a death domain and both have been shown to act mainly through TRAF3 and TRAF6 to activate the NF-κB and JNK pathways. Cells transfected with XEDAR and treated with EDA-A2 cause the assembly of a secondary complex containing FADD, caspase-8 and caspase-10, leading to the activation caspase-8 and caspase-3, and finally apoptosis. The EDA-A2-induced apoptosis is dependent on caspase-9 activation, as various pharmacological and genetic inhibitors of caspase-8 blocked apoptosis following EDA-A2 treatment.

NCBI and Uniprot Product Information

• NCBI GI #: 37222208
• NCBI GeneID: 60401
• UniProt Accession #: Q9HAV5; Q5VYX9; Q5VYY0; Q6UWM2; Q8IZA6
• Molecular Weight: 32,959 Da
• NCBI Official Full Name: XEDAR
• NCBI Official Synonym Full Names: ectodysplasin A2 receptor
• NCBI Official Symbol: EDA2R
• NCBI Official Synonym Symbols: XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 27; EDA-A2 receptor; X-linked ectodysplasin-A2 receptor; tumor necrosis factor receptor superfamily member XEDAR
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 27
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: EDA2R
• UniProt Synonym Gene Names: TNFRSF27; XEDAR; EDA-A2 receptor
• UniProt Entry Name: TNR27_HUMAN

NCBI Description

EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

EDA2R: Receptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: integral to plasma membrane; integral to membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity; receptor activity

Biological Process: epidermis development; tumor necrosis factor-mediated signaling pathway; multicellular organismal development; positive regulation of JNK cascade; activation of NF-kappaB transcription factor