Rabbit anti-Human SDS Polyclonal Antibody | anti-SDS antibody

SDS, NT (SDS, SDH, L-serine dehydratase/L-threonine deaminase, L-serine deaminase, L-threonine dehydratase) (PE)

Cat. Num. AAA6345689

• Gene Names: SDS; SDH
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SDS; Polyclonal Antibody; NT (SDS; SDH; L-serine dehydratase/L-threonine deaminase; L-serine deaminase; L-threonine dehydratase) (PE); L-threonine dehydratase; anti-SDS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SDS antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SDS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human SDS.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SDS Antibody (N-term) in K562 cell line lysates (35ug/lane). SDS (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(SDS Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SDS antibody

SDS encodes one of three enzymes that are involved in metabolizing serine and glycine. L-serine dehydratase converts L-serine to pyruvate and ammonia and requires pyridoxal phosphate as a cofactor. The encoded protein can also metabolize threonine to NH4+ and 2-ketobutyrate. The encoded protein is found predominantly in the liver.

Product Categories/Family for anti-SDS antibody

Antibodies; Enzymes

References

Yamada, T., et.al., Biochim. Biophys. Acta 1780 (5), 809-818 (2008). Szaflik, J.P., et.al., Exp. Eye Res. 86 (4), 647-652 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 18088452
• NCBI GeneID: 10993
• UniProt Accession #: P20132; A8K9P5
• Molecular Weight: 34,625 Da
• NCBI Official Full Name: SDS protein
• NCBI Official Synonym Full Names: serine dehydratase
• NCBI Official Symbol: SDS
• NCBI Official Synonym Symbols: SDH
• NCBI Protein Information: L-serine dehydratase/L-threonine deaminase; TDH; L-serine deaminase; L-serine ammonia-lyase; L-threonine dehydratase
• UniProt Protein Name: L-serine dehydratase/L-threonine deaminase
• Protein Family: SDS degradation transcriptional activation protein
• UniProt Gene Name: SDS
• UniProt Synonym Gene Names: SDH; SDH; TDH
• UniProt Entry Name: SDHL_HUMAN

Uniprot Description

Catalytic activity: L-serine = pyruvate + NH3. Ref.4L-threonine = 2-oxobutanoate + NH3. Ref.4

Cofactor: Pyridoxal phosphate.

Pathway: Carbohydrate biosynthesis; gluconeogenesis.

Subunit structure: Homodimer.

Subcellular location: Cytoplasm.

Sequence similarities: Belongs to the serine/threonine dehydratase family.

Biophysicochemical propertiesKinetic parameters:KM=23 mM for serine Ref.4KM=31 mM for threonine