Rabbit anti-Human WTIP Polyclonal Antibody | anti-WTIP antibody

WTIP, CT (WTIP, Wilms tumor protein 1-interacting protein)

Cat. Num. AAA645843

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: WTIP; Polyclonal Antibody; CT (WTIP; Wilms tumor protein 1-interacting protein); Anti -WTIP; anti-WTIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-WTIP antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Immunofluorescence: 1:10-50
• Immunogen: WTIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human WTIP.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(WTIP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WTIP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(WTIP Antibody (C-term) western blot analysis in NCI-H460,WiDr cell line lysates (35ug/lane).This demonstrates the WTIP antibody detected the WTIP protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of WTIP Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-WTIP antibody

WTIP may monitor slit diaphragm protein assembly, a specialized adherens junction characteristic of podocytes. In case of podocyte injury, it shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype (By similarity).

Product Categories/Family for anti-WTIP antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 122937269
• NCBI GeneID: 126374
• NCBI Accession #: NP_001073905.1
• NCBI GenBank Nucleotide #: NM_001080436.1
• UniProt Accession #: A6NIX2
• Molecular Weight: 45,124 Da
• NCBI Official Full Name: Wilms tumor protein 1-interacting protein
• NCBI Official Synonym Full Names: Wilms tumor 1 interacting protein
• NCBI Official Symbol: WTIP
• NCBI Protein Information: Wilms tumor protein 1-interacting protein; WT1-interacting protein
• UniProt Protein Name: Wilms tumor protein 1-interacting protein
• Protein Family: Wilms tumor protein 1-interacting protein
• UniProt Gene Name: WTIP
• UniProt Synonym Gene Names: WT1-interacting protein
• UniProt Entry Name: WTIP_HUMAN

Uniprot Description

Function: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates Hippo signaling pathway and antagonizes phosphorylation of YAP1. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. In podocytes, may play a role in the regulation of actin dynamics and/or foot process cytoarchitecture

By similarity. In the course of podocyte injury, shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype. Ref.2 Ref.4 Ref.5 Ref.6

Subunit structure: Forms homodimers

By similarity. Interacts with CD2AP and WT1. Interacts (via LIM domains) with SNAI1 (via SNAG domain), SNAI2/SLUG (via SNAG domain) and SCRT1 (via SNAG domain)

By similarity. Interacts with EIF4E, AGO1, AGO2, DCP2, DDX6, LATS1, LATS2, SAV1, EGLN2/PHD1 and EGLN3/PHD3. Interacts (via LIM domains) with isoform 1 and isoform 3 of VHL. Interacts with ROR2

By similarity. Following treatment with bacterial lipopolysaccharide (LPS), forms a complex with MAPK8IP3 and dynein intermediate chain

By similarity. Ref.2 Ref.4 Ref.6

Subcellular location: Cell junction › adherens junction. Nucleus

By similarity. Cytoplasm › P-body. Note: Following podocyte injury, caused by treatment with LPS, puromycin aminonucleoside, ultraviolet or hydrogen peroxide, translocates from sites of cell-cell contacts into the cytosol and nucleus. The shift from cell contacts to intracellular plaques starts as early as 1 hour after LPS stimulation and intranuclear localization begins 3 hours after LPS treatment. Maximal nuclear localization is achieved 6 hours after LPS treatment. Nuclear translocation requires dynein motor activity and intact microtubule network

By similarity. Returns to cell-cell contacts 24 hours after LPS stimulation. In the presence of ROR2, localizes to the plasma membrane

By similarity. Ref.3 Ref.4

Sequence similarities: Belongs to the zyxin/ajuba family.Contains 3 LIM zinc-binding domains.