Rabbit anti-Human IGF2BP1 Polyclonal Antibody | anti-IGF2BP1 antibody

IGF2BP1, CT (IGF2BP1, CRDBP, VICKZ1, ZBP1, Insulin-like growth factor 2 mRNA-binding protein 1, Coding region determinant-binding protein, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zip code-binding protein 1) (FITC)

Cat. Num. AAA6309478

• Gene Names: IGF2BP1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IGF2BP1; Polyclonal Antibody; CT (IGF2BP1; CRDBP; VICKZ1; ZBP1; Insulin-like growth factor 2 mRNA-binding protein 1; Coding region determinant-binding protein; IGF-II mRNA-binding protein 1; VICKZ family member 1; Zip code-binding protein 1) (FITC); Zip code-binding protein 1; anti-IGF2BP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-IGF2BP1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IGF2BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 515-542 amino acids from the C-terminal region of human IGF2BP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IGF2BP1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the IGF2BP1 antibody detected the IGF2BP1 protein (arrow).)

Immunohistochemistry (IHC)

(IGF2BP1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF2BP1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(IGF2BP1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-IGF2BP1 antibody

IGF2BP1 is a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-IGF2BP1 antibody

Antibodies; Nucleic Acids

References

Rodriguez, S., et al. Growth Horm. IGF Res. 20(4):310-318(2010). Pillas, D., et al. PLoS Genet. 6 (2), E1000856 (2010): Noubissi, F.K., et al. Cancer Res. 69(22):8572-8578(2009). Zhou, Y., et al. Virology 393(2):210-220(2009). Kawakami, Y., et al. Biosci. Biotechnol. Biochem. 73(8):1811-1817(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 56237027
• NCBI GeneID: 10642
• NCBI Accession #: NP_006537.3
• NCBI GenBank Nucleotide #: NM_006546.3
• UniProt Accession #: Q9NZI8; C9JT33
• Molecular Weight: 48,598 Da
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: insulin-like growth factor 2 mRNA binding protein 1
• NCBI Official Symbol: IGF2BP1
• NCBI Official Synonym Symbols: IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 1; ZBP-1; VICKZ family member 1; zipcode-binding protein 1; IGF2 mRNA-binding protein 1; IGF-II mRNA-binding protein 1; coding region determinant-binding protein
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 1
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP1
• UniProt Synonym Gene Names: CRDBP; VICKZ1; ZBP1; IGF2 mRNA-binding protein 1; IMP-1; IMP1; CRD-BP; ZBP-1
• UniProt Entry Name: IF2B1_HUMAN

NCBI Description

This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

IGF2BP1: a conserved trans-acting factor that can control translation of specific mRNAs in vivo. Contains four K homology (KH) domains and two RNA recognition motifs. It functions by binding to the 5' UTR of the target mRNAs and repressing translation. Target mRNAs include those encoding beta-actin and insulin-like growth factor 2 (IGF2). Tyrosine phosphorylation apparently abolishes translational repression of actin expression by decreasing its binding beta-actin mRNA. May have a role in progression of ovarian cancer. Contains nuclear export signals within the RNA-binding KH2 and KH4 domains. Aberrant expression may interfere with c-myc regulation.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: growth cone; perinuclear region of cytoplasm; stress granule; axon; lamellipodium; cytoplasm; dendritic spine; ribonucleoprotein complex; cytosol; nucleus; filopodium

Molecular Function: mRNA binding; protein binding; mRNA 3'-UTR binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: mRNA transport; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process