Rabbit WFS1 Polyclonal Antibody | anti-WFS1 antibody

WFS1 (Wolfram Syndrome 1, FLJ51211, WFRS, WFS, Wolframin) (HRP)

Cat. Num. AAA6443396

• Gene Names: WFS1; WFS; WFRS; WFSL; CTRCT41
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: WFS1; Polyclonal Antibody; WFS1 (Wolfram Syndrome 1; FLJ51211; WFRS; WFS; Wolframin) (HRP); anti-WFS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes WFS1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-WFS1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human WFS1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of mouse brain cell lines, using WFS1 antibody.)

Product Categories/Family for anti-WFS1 antibody

Antibodies; Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 119602804
• NCBI GeneID: 7466
• UniProt Accession #: O76024; Q8N6I3; Q9UNW6; B2R797; D3DVT1
• Molecular Weight: 100,292 Da
• NCBI Official Full Name: Wolfram syndrome 1 (wolframin), isoform CRA_a
• NCBI Official Synonym Full Names: Wolfram syndrome 1 (wolframin)
• NCBI Official Symbol: WFS1
• NCBI Official Synonym Symbols: WFS; WFRS; WFSL; CTRCT41
• NCBI Protein Information: wolframin
• UniProt Protein Name: Wolframin
• Protein Family: Wolframin
• UniProt Gene Name: WFS1
• UniProt Entry Name: WFS1_HUMAN

NCBI Description

This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

WFS1: Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store. Highly expressed in heart followed by brain, placenta, lung and pancreas. Weakly expressed in liver, kidney and skeletal muscle. Also expressed in islet and beta-cell insulinoma cell line.

Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p16.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; dendrite; integral to endoplasmic reticulum membrane

Molecular Function: calmodulin binding; transcription activator binding; transporter activity; ubiquitin protein ligase binding; calcium-dependent protein binding; ATPase binding

Biological Process: protein maturation via protein folding; ER-associated protein catabolic process; olfactory behavior; protein stabilization; positive regulation of proteolysis; unfolded protein response; negative regulation of transcription factor activity; glucose homeostasis; positive regulation of protein metabolic process; neurological system process; calcium ion homeostasis; endoplasmic reticulum calcium ion homeostasis; unfolded protein response, activation of signaling protein activity; sensory perception of sound; cellular protein metabolic process; visual perception; positive regulation of protein ubiquitination; negative regulation of programmed cell death; renal water homeostasis; negative regulation of neuron apoptosis; kidney development; positive regulation of calcium ion transport; positive regulation of growth; ER overload response

Disease: Deafness, Autosomal Dominant 6; Wolfram Syndrome 1; Wolfram-like Syndrome, Autosomal Dominant; Cataract 41; Diabetes Mellitus, Noninsulin-dependent