Western Blot (WB)
(Western blot analysis of DNAJC2 Antibody (Center) in mouse Neuro-2a cell line lysates (35ug/lane). DNAJC2 (arrow) was detected using the purified Pab.)
Rabbit DNAJC2 Polyclonal Antibody | anti-DNAJC2 antibody
DNAJC2 Antibody (Center)
Western Blot (WB)
(Western blot analysis of DNAJC2 Antibody (Center) in mouse Neuro-2a cell line lysates (35ug/lane). DNAJC2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DNAJC2 Antibody (Center) in mouse Neuro-2a cell line lysates (35ug/lane). DNAJC2 (arrow) was detected using the purified Pab.)
Function:
Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Otto, H., et al. Proc. Natl. Acad. Sci. U.S.A. 102(29):10064-10069(2005)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
DNAJC2: Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; DNA replication; Chaperone
Chromosomal Location of Human Ortholog: 7q22
Cellular Component: nuclear membrane; cytoplasm; nucleus; cytosol
Molecular Function: ubiquitin binding; DNA binding; histone binding; chromatin binding; Hsp70 protein binding
Biological Process: 'de novo' cotranslational protein folding; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; chromatin modification; negative regulation of cell growth; DNA replication
Research Articles on DNAJC2
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Product Notes
The DNAJC2 dnajc2 (Catalog #AAA9213732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 226-255. The DNAJC2 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine, Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the DNAJC2 dnajc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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