Western Blot (WB)
(WDR85 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the WDR85 antibody detected the WDR85 protein (arrow).)
Rabbit anti-Human WDR85 Polyclonal Antibody | anti-DPH7 antibody
WDR85, NT (WDR85, C9orf112, WD repeat-containing protein 85)
Gene Names
DPH7; RRT2; WDR85; C9orf112; RP11-48C7.3
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
WDR85; Polyclonal Antibody; NT (WDR85; C9orf112; WD repeat-containing protein 85); Anti -WDR85; anti-DPH7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-DPH7 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
WDR85 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-51 amino acids from the N-terminal region of human WDR85.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WDR85 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the WDR85 antibody detected the WDR85 protein (arrow).)
Western Blot (WB)
(WDR85 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the WDR85 antibody detected the WDR85 protein (arrow).)
Related Product Information for anti-DPH7 antibody
WDR85 is a WD repeat-containing protein that plays a role in the first step of diphthamide biosynthesis (Carette et al., 2009 [PubMed 19965467]).
Product Categories/Family for anti-DPH7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,575 Da
NCBI Official Full Name
WDR85 protein
NCBI Official Synonym Full Names
diphthamide biosynthesis 7
NCBI Official Symbol
DPH7
NCBI Official Synonym Symbols
RRT2; WDR85; C9orf112; RP11-48C7.3
NCBI Protein Information
diphthamide biosynthesis protein 7; WD repeat domain 85; WD repeat-containing protein 85
UniProt Protein Name
Diphthamide biosynthesis protein 7
Protein Family
UniProt Gene Name
DPH7
UniProt Synonym Gene Names
C9orf112; WDR85
UniProt Entry Name
DPH7_HUMAN
NCBI Description
WDR85 is a WD repeat-containing protein that plays a role in the first step of diphthamide biosynthesis (Carette et al., 2009 [PubMed 19965467]).[supplied by OMIM, Feb 2010]
Uniprot Description
WDR85: Plays a role in the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 which can be ADP- ribosylated by diphtheria toxin and by Pseudomonas exotoxin A. Belongs to the WD repeat WDR85 family.
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: cytosol
Biological Process: cellular protein metabolic process; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; post-translational protein modification
Research Articles on DPH7
Similar Products
Product Notes
The DPH7 dph7 (Catalog #AAA643109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WDR85, NT (WDR85, C9orf112, WD repeat-containing protein 85) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WDR85 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the DPH7 dph7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR85, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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