Western Blot (WB)
(Western blot of human Jurkat cells (lanes 1-3). The blots were probed with anti-WASH (C-terminal region) rabbit polyclonal antibody at 1:250 (lane 1) or at 1:1000 in the absence (lane 2) or presence of WASH blocking peptide (WX4005) (lane 3).)
Rabbit WASH Polyclonal Antibody | anti-WASH antibody
WASH (C-terminal region) Antibody
Gene Names
WASHC1; WASH; WASH1; FAM39E
Reactivity
Human, Rat, Mouse, Chicken
Applications
Western Blot, ELISA, Immunocytochemistry
Synonyms
WASH; Polyclonal Antibody; WASH (C-terminal region) Antibody; WASH (C-terminal region); anti-WASH antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken
Clonality
Polyclonal
Specificity
This antibody was affinity purified using WASH (C-terminal region) peptide (without carrier). The antibody detects a 70kDa* protein corresponding to the molecular mass of WASH on SDS-PAGE immunoblots of human Jurkat cells.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-WASH antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:2000
ICC: 1:200
WB: 1:1000
ICC: 1:200
WB: 1:1000
Immunogen
WASH synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human WASH. This sequence is highly conserved in rat, mouse, and chicken WASH, and has low homology to other WASP family members.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of human Jurkat cells (lanes 1-3). The blots were probed with anti-WASH (C-terminal region) rabbit polyclonal antibody at 1:250 (lane 1) or at 1:1000 in the absence (lane 2) or presence of WASH blocking peptide (WX4005) (lane 3).)
Western Blot (WB)
(Western blot of human Jurkat cells (lanes 1-3). The blots were probed with anti-WASH (C-terminal region) rabbit polyclonal antibody at 1:250 (lane 1) or at 1:1000 in the absence (lane 2) or presence of WASH blocking peptide (WX4005) (lane 3).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of WASH relative to F-actin in chick DRG neurons. The cells were labeled with rabbit polyclonal WASH (C-terminal region) antibody (WP4001), then the antibody was detected using appropriate secondary antibody (Green). On the left, this WASH labeling is compared to F-actin staining (Red). (Image provided by Dr. Gianluca Gallo at Drexel University).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of WASH relative to F-actin in chick DRG neurons. The cells were labeled with rabbit polyclonal WASH (C-terminal region) antibody (WP4001), then the antibody was detected using appropriate secondary antibody (Green). On the left, this WASH labeling is compared to F-actin staining (Red). (Image provided by Dr. Gianluca Gallo at Drexel University).)
Related Product Information for anti-WASH antibody
The Wiskott-Aldrich syndrome protein (WASP) family functions downstream of Rho GTPases to mediate the branched-actin network formation required for cytoskeletal remodeling, intracellular transport, and cell motility. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms (WAVE1, 2, and 3). Two new WASP subfamilies are WASH and WHAMM. WASH is an F-actin binding protein that can promote Arp2/3-mediated branch actin nucleation. The WASH protein contains two WASH homology domains, a proline-rich region, and a C-terminal VCA domain. WASH can interact with dynamin, FAM21, and tubulin, and WASH activity may be regulated downstream of Rho GTPases. WASH interaction with FAM21 has been implicated in endosome sorting, and inactivation of WASH leads to endosome tubulation. Thus, WASH may be a critical element in the regulation of the actin filament and microtubule networks that mediate endosomal sorting and fission.
References
Derivery, E. et al. (2009) Dev. Cell. 17:712.
Gomez, T.S. & Billadeau, D.D. (2009) Dev. Cell. 17:699.
Liu, R. et al. (2009) Development. 136:2849.
Linardopoulou, E.V. et al. (2007) PLoS Genet. 3:e237.
Gomez, T.S. & Billadeau, D.D. (2009) Dev. Cell. 17:699.
Liu, R. et al. (2009) Development. 136:2849.
Linardopoulou, E.V. et al. (2007) PLoS Genet. 3:e237.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,328 Da
NCBI Official Full Name
WAS protein family homolog 1
NCBI Official Synonym Full Names
WASH complex subunit 1
NCBI Official Symbol
WASHC1
NCBI Official Synonym Symbols
WASH; WASH1; FAM39E
NCBI Protein Information
WAS protein family homolog 1
UniProt Protein Name
WAS protein family homolog 1
Protein Family
UniProt Gene Name
WASH1
UniProt Synonym Gene Names
FAM39E
UniProt Entry Name
WASH1_HUMAN
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Product Notes
The WASH wash1 (Catalog #AAA474653) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WASH (C-terminal region) Antibody reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's WASH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:200 WB: 1:1000. Researchers should empirically determine the suitability of the WASH wash1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WASH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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