Rabbit WAVE1 Polyclonal Antibody | anti-WAVE1 antibody

WAVE1 (I119) Polyclonal Antibody

Cat. Num. AAA3001387

• Gene Names: WASF1; WAVE; SCAR1; WAVE1; NEDALVS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: WAVE1; Polyclonal Antibody; WAVE1 (I119) Polyclonal Antibody; Wiskott-Aldrich syndrome protein family member 1; WASP family protein member 1; Protein WAVE-1; Verprolin homology domain-containing protein 1; WASF1; KIAA0269; SCAR1; anti-WAVE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: WAVE1 (I119) polyclonal antibody detects endogenous levels of WAVE1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 559

• Applicable Applications for anti-WAVE1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 88-132 of Human WAVE1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of WAVE1 (I119) pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:The Brain tissue lysate of Rat (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:Hela whole cell lysate (40ug) Lane5:L02 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of WAVE1 (I119) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-WAVE1 antibody

WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 68161500
• NCBI GeneID: 8936
• NCBI Accession #: NP_001020105.1
• NCBI GenBank Nucleotide #: NP_001020105.1
• UniProt Accession #: Q92558
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: wiskott-Aldrich syndrome protein family member 1
• NCBI Official Synonym Full Names: WASP family member 1
• NCBI Official Symbol: WASF1
• NCBI Official Synonym Symbols: WAVE; SCAR1; WAVE1; NEDALVS
• NCBI Protein Information: wiskott-Aldrich syndrome protein family member 1
• UniProt Protein Name: Wiskott-Aldrich syndrome protein family member 1
• UniProt Gene Name: WASF1
• UniProt Synonym Gene Names: KIAA0269; SCAR1; WAVE1; WASP family protein member 1
• UniProt Entry Name: WASF1_HUMAN

NCBI Description

The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WAVE1: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Belongs to the SCAR/WAVE family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoskeleton; synapse; cell junction; actin cytoskeleton

Molecular Function: protein binding; Rac GTPase binding; protein complex binding; actin binding

Biological Process: actin filament polymerization; Rac protein signal transduction; protein complex assembly; cell motility