Western Blot (WB)
(Western blot analysis of WAPL in A20 cell lysate with WAPL antibody at (A) 1 and (B) 2 μg/ml.)
Rabbit WAPL Polyclonal Antibody | anti-WAPAL antibody
WAPL Antibody
Gene Names
WAPAL; FOE; WAPL; KIAA0261
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
WAPL antibody is affinity chromatography purified via peptide column.
Synonyms
WAPL; Polyclonal Antibody; WAPL Antibody; FOE; KIAA0261; Wings apart-like protein homolog; Friend of EBNA2 protein; wings apart-like homolog (Drosophila); anti-WAPAL antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist.
Purity/Purification
WAPL antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1190
Applicable Applications for anti-WAPAL antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry at 5 mug/mL. For Immunoflorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
WAPL antibody was raised against an 18 amino acid peptide near the amino terminus of human WAPL.
Buffer
WAPL antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
WAPL antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of WAPL in A20 cell lysate with WAPL antibody at (A) 1 and (B) 2 μg/ml.)
Western Blot (WB)
(Western blot analysis of WAPL in A20 cell lysate with WAPL antibody at (A) 1 and (B) 2 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of WAPL in rat heart tissue with WAPL antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of WAPL in rat heart tissue with WAPL antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of WAPL in rat heart tissue with WAPL antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of WAPL in rat heart tissue with WAPL antibody at 20 μg/mL.)
Related Product Information for anti-WAPAL antibody
WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1). It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1,2,4). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3,4). WAPL is a new regulator of the development and metastasis of cancerous tissue (4,5).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 131 kDa
Observed: 140 kDa
Observed: 140 kDa
NCBI Official Full Name
wings apart-like protein homolog
NCBI Official Synonym Full Names
wings apart-like homolog (Drosophila)
NCBI Official Symbol
WAPAL
NCBI Official Synonym Symbols
FOE; WAPL; KIAA0261
NCBI Protein Information
wings apart-like protein homolog; friend of EBNA2 protein; friend of EBNA2 (Epstein-Barr virus nuclear protein 2)
UniProt Protein Name
Wings apart-like protein homolog
UniProt Gene Name
WAPAL
UniProt Synonym Gene Names
FOE; KIAA0261; WAPL
UniProt Entry Name
WAPL_HUMAN
Uniprot Description
WAPL: a chromatin-associated protein partner of Epstein-Barr nuclear protein 2. Associated with cervical carcinogenesis and tumor progression.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 10q23.2
Cellular Component: cohesin complex; microtubule cytoskeleton; nucleoplasm; intracellular membrane-bound organelle; cytoplasm; synaptonemal complex; chromosome; cytosol; nucleus; chromatin; chromosome, pericentric region
Molecular Function: protein binding
Biological Process: mitosis; positive regulation of fibroblast proliferation; viral reproduction; cell division; negative regulation of DNA replication; response to toxin; negative regulation of sister chromatid cohesion; mitotic cell cycle
Research Articles on WAPAL
Similar Products
Product Notes
The WAPAL wapal (Catalog #AAA153367) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WAPL Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WAPL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry at 5 mug/mL. For Immunoflorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the WAPAL wapal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WAPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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