FBXO2 recombinant protein

FBXO2, 1-296aa, Human, His tag, E Coli

Cat. Num. AAA205630

• Gene Names: FBXO2; FBG1; FBX2; Fbs1; OCP1; NFB42
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: FBXO2; 1-296aa; Human; His tag; E Coli; F-box only protein 2; FBG1; Fbs1; FBX2; NFB42; OCP1; FBXO2 recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDGDGDP ESVGQPEEAS PEEQPEEASA EEERPEDQQE EEAAAAAAYL DELPEPLLLR VLAALPAAEL VQACRLVCLR WKELVDGAPL WLLKCQQEGL VPEGGVEEER DHWQQFYFLS KRRRNLLRNP CGEEDLEGWC DVEHGGDGWR VEELPGDSGV EFTHDESVKK YFASSFEWCR KAQVIDLQAE GYWEELLDTT QPAIVVKDWY SGRSDAGCLY ELTVKLLSEH ENVLAEFSSG QVAVPQDSDG GGWMEISHTF TDYGPGVRFV RFEHGGQDSV YWKGWFGARV TNSSVWVEP
• Sequence Length: 296

• Applicable Applications for FBXO2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for FBXO2 recombinant protein

FBXO2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein belongs to the Fbxs class. It is highly similar to the rat NFB42 (neural F Box 42 kDa) protein which is enriched in the nervous system and may play a role in maintaining neurons in a postmitotic state. Recombinant human FBXO2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques

Product Categories/Family for FBXO2 recombinant protein

Ubiquitination

References

Cenciarelli C., et al (1999). Curr. Biol. 9:1177-1179; Burkard T.R., et al (2011). BMC Syst. Biol. 5:17-17;

NCBI and Uniprot Product Information

• NCBI GI #: 15812198
• NCBI GeneID: 26232
• NCBI Accession #: NP_036300
• NCBI GenBank Nucleotide #: NP_036300.2
• UniProt Accession #: Q9UK22; Q5TGY0; Q6FGJ7; Q8TB29; Q9UKC6; B2R7K7
• Molecular Weight: 35.7 kDa (319aa), confirmed by MALDI-TOF
• NCBI Official Full Name: F-box only protein 2
• NCBI Official Synonym Full Names: F-box protein 2
• NCBI Official Symbol: FBXO2
• NCBI Official Synonym Symbols: FBG1; FBX2; Fbs1; OCP1; NFB42
• NCBI Protein Information: F-box only protein 2
• UniProt Protein Name: F-box only protein 2
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO2
• UniProt Synonym Gene Names: FBX2

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is highly similar to the rat NFB42 (neural F Box 42 kDa) protein which is enriched in the nervous system and may play a role in maintaining neurons in a postmitotic state. [provided by RefSeq, Jul 2008]

Uniprot Description

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type ().