Rabbit anti-Mouse, Rat VPS34 Polyclonal Antibody | anti-Pik3c3 antibody

RABBIT ANTI VPS34

Cat. Num. AAA224363

• Gene Names: Pik3c3; Vps34; 5330434F23Rik
• Reactivity: Mouse, Rat
• Applications: Immunofluorescence, Western Blot
• Synonyms: VPS34; Polyclonal Antibody; RABBIT ANTI VPS34; anti-Pik3c3 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises VPS34 (vacuolar protein sorting 34), otherwise known as PI3-kinase type 3 (PIK3C3), a ubiquitously expressed class III PI3K (phosphoinositide 3-kinase), and member of the PI3/PI4-kinase family, which specifically utilizes phosphatidylinositol (PtdIn) as a substrate, and functions as the catalytic subunit of the PI3K complex. ; VPS34 plays a role in the transport of lysosomal enzyme precursors to lysosomes, and in endocytosis and vesicular trafficking, and has been strongly implicated as a regulator of autophagy, forming a multi-protein complex with the proautophagic tumor suppressors Beclin1/Atg6, Bif-1, and UVRAG, in the initiation of autophagosome formation. Distinct complexes of VPS34 have been shown to regulate endocytic processes vital for late-stage autophagosome-lysosome fusion. ; This item has been tested in immunofluorescence using HUVEC cells, in conjunction with Hoescht 33342 (blue) nuclear staining.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 887

• Applicable Applications for anti-Pik3c3 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 105kDa in HEK-293T cell lysates.; Immunofluorescence: Minimum Dilution: 1/100; Western Blotting: Minimum Dilution: 2.0; Maximum Dilution: 4.0ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: KLH conjugated synthetic peptide corresponding to amino acids 22-34 of human VPS34, The corresponding sequence is identical in mouse and rat.; Buffer Solution
• Antiserum Preparation: Antiserum to human VSP34 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-Pik3c3 antibody

MBS224363 specifically recognises VPS34 (vacuolar protein sorting 34), otherwise known as PI3-kinase type 3 (PIK3C3), a ubiquitously expressed class III PI3K (phosphoinositide 3-kinase), and member of the PI3/PI4-kinase family, which specifically utilizes phosphatidylinositol (PtdIn) as a substrate, and functions as the catalytic subunit of the PI3K complex. VPS34 plays a role in the transport of lysosomal enzyme precursors to lysosomes, and in endocytosis and vesicular trafficking, and has been strongly implicated as a regulator of autophagy, forming a multi-protein complex with the proautophagic tumor suppressors Beclin1/Atg6, Bif-1, and UVRAG, in the initiation of autophagosome formation. Distinct complexes of VPS34 have been shown to regulate endocytic processes vital for late-stage autophagosome-lysosome fusion. MBS224363 has been tested in immunofluorescence using HUVEC cells, in conjunction with Hoescht 33342 (blue) nuclear staining.

NCBI and Uniprot Product Information

• NCBI GI #: 42475974
• NCBI GeneID: 225326
• NCBI Accession #: NP_852079.2
• NCBI GenBank Nucleotide #: NM_181414.5
• Molecular Weight: 96,869 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase catalytic subunit type 3
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, class 3
• NCBI Official Symbol: Pik3c3
• NCBI Official Synonym Symbols: Vps34; 5330434F23Rik
• NCBI Protein Information: phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; ptdIns-3-kinase type 3
• UniProt Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• UniProt Gene Name: Pik3c3
• UniProt Synonym Gene Names: PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3
• UniProt Entry Name: PK3C3_MOUSE

Uniprot Description

PIK3C3: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Ubiquitously expressed, with a highest expression in skeletal muscle. Belongs to the PI3/PI4-kinase family.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.137; Kinase, lipid; Autophagy; Motility/polarity/chemotaxis

Cellular Component: membrane; late endosome; phagocytic vesicle; midbody; phosphoinositide 3-kinase complex; endosome

Molecular Function: transferase activity; protein binding; 1-phosphatidylinositol-3-kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; phosphotransferase activity, alcohol group as acceptor; phosphoinositide 3-kinase activity; ATP binding; protein kinase activity

Biological Process: phosphoinositide-mediated signaling; endosome organization and biogenesis; cytokinesis; cell cycle; protein amino acid phosphorylation; regulation of protein secretion; early endosome to late endosome transport; phosphoinositide phosphorylation; cell division; autophagy; protein processing; phosphorylation; autophagic vacuole formation