Rabbit PI3 Kinase Class III Polyclonal Antibody | anti-Pik3c3 antibody

PI3 Kinase Class III Antibody

Cat. Num. AAA9611209

• Gene Names: PIK3C3; VPS34; Vps34; hVps34
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PI3 Kinase Class III; Polyclonal Antibody; PI3 Kinase Class III Antibody; hVps34; MGC61518; Phosphatidylinositol 3 kinase catalytic subunit type 3; Phosphatidylinositol 3 kinase class 3; Phosphatidylinositol 3 kinase p100 subunit; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide 3 kinase class 3; Phosphoinositide-3-kinase class 3; PI3 kinase type 3; PI3-kinase type 3; PI3K type 3; PK3C3_HUMAN; PtdIns 3 kinase type 3; PtdIns-3-kinase type 3; Vps 34; Vps34; anti-Pik3c3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PI3 Kinase Class III Antibody detects endogenous levels of PI3 Kinase Class III.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 887

• Applicable Applications for anti-Pik3c3 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PI3 Kinase Class III.
• Tissue Specificity: Ubiquitously expressed, with a highest expression in skeletal muscle.
• Subcellular Location: Endosome
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of PI3 Kinase Class III in lysates of 293T?, using PI3 Kinase Class III Antibody.)

Related Product Information for anti-Pik3c3 antibody

Description: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).

Subunit Structure: Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are: the PI3K complex I (PI3KC3-C1) containing ATG14, and the PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits such as RUBCN, SH3GLB1/Bif-1 and AMBRA1 (PubMed:7628435, PubMed:19050071, PubMed:20643123, PubMed:19270696, PubMed:23878393, PubMed:25490155). PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3R4 (PubMed:14617358). Interacts with AMBRA1 (By similarity). Interacts with BECN1P1/BECN2 (PubMed:23954414). Interacts with SLAMF1(PubMed:22493499). May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3 (By similarity). Interacts with NCKAP1L (PubMed:16417406).

Similarity: Belongs to the PI3/PI4-kinase family.

NCBI and Uniprot Product Information

• NCBI GI #: 34761064
• NCBI GeneID: 5289
• NCBI Accession #: NP_002638.2
• NCBI GenBank Nucleotide #: NP_002638.2
• UniProt Accession #: Q8NEB9
• Molecular Weight: Observed: 100 kDa; Predicted: 102 kDa
• NCBI Official Full Name: phosphatidylinositol 3-kinase catalytic subunit type 3 isoform 1
• NCBI Official Synonym Full Names: phosphatidylinositol 3-kinase catalytic subunit type 3
• NCBI Official Symbol: PIK3C3
• NCBI Official Synonym Symbols: VPS34; Vps34; hVps34
• NCBI Protein Information: phosphatidylinositol 3-kinase catalytic subunit type 3
• UniProt Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• UniProt Gene Name: PIK3C3
• UniProt Synonym Gene Names: VPS34; PI3-kinase type 3; PI3K type 3
• UniProt Entry Name: PK3C3_HUMAN