Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF14/HVEM PicoKine ELISA Kit standard curve )
Mouse TNFRSF14/HVEM ELISA Kit | TNFRSF14 elisa kit
Mouse TNFRSF14/HVEM PicoKine ELISA Kit
Gene Names
Tnfrsf14; Atar; HveA; Hvem; Tnfrs14
Reactivity
Mouse
Synonyms
TNFRSF14/HVEM; Mouse TNFRSF14/HVEM PicoKine ELISA Kit; Protein Tnfrsf14; tumor necrosis factor receptor superfamily; member 14; CD270; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; TNFRSF14; TNR14_HUMAN; TR2; Tumor necrosis factor receptor superfamily member 14; Tumor necrosis factor receptor-like 2; TNFRSF14 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse TNFRSF14
Sequence Length
275
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF14/HVEM PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF14/HVEM PicoKine ELISA Kit standard curve )
Related Product Information for TNFRSF14 elisa kit
Principle of the assay: mouse TNFRSF14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF14 has been precoated onto 96-well plates. Standards(CHO, Q29-V207) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF14 amount of sample captured in plate.
Background: Tumor necrosis factor receptor superfamily member 14 (TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.
Background: Tumor necrosis factor receptor superfamily member 14 (TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
30,171 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 14
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
NCBI Official Symbol
Tnfrsf14
NCBI Official Synonym Symbols
Atar; HveA; Hvem; Tnfrs14
NCBI Protein Information
tumor necrosis factor receptor superfamily member 14; herpes virus entry mediator
UniProt Protein Name
Protein Tnfrsf14
Protein Family
UniProt Gene Name
Tnfrsf14
UniProt Entry Name
Q80WM9_MOUSE
Research Articles on TNFRSF14
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Product Notes
The Mouse TNFRSF14 tnfrsf14 (Catalog #AAA177206) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177206 ELISA Kit recognizes Mouse TNFRSF14. It is sometimes possible for the material contained within the vial of "TNFRSF14/HVEM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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