Rabbit VPS33B Polyclonal Antibody | anti-VPS33B antibody

VPS33B Rabbit Polyclonal

Cat. Num. AAA7605669

• Reactivity: Human, Mouse, Rat, Zebrafish
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: VPS33B; Polyclonal Antibody; VPS33B Rabbit Polyclonal; anti-VPS33B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 617

• Applicable Applications for anti-VPS33B antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Vacuolar protein sorting 33 homolog B (yeast)
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS7605669 (VPS33B antibody) at dilution of 1:100)

Western Blot (WB)

(mouse testis tissue were subjected to SDS PAGE followed by western blot with MBS7605669 (VPS33B antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 119829191
• NCBI GeneID: 26276
• NCBI Accession #: NP_061138.3
• NCBI GenBank Nucleotide #: NM_018668.4
• Molecular Weight: 60,032 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 33B isoform 1
• NCBI Official Synonym Full Names: VPS33B, late endosome and lysosome associated
• NCBI Official Symbol: VPS33B
• NCBI Protein Information: vacuolar protein sorting-associated protein 33B
• UniProt Protein Name: Vacuolar protein sorting-associated protein 33B
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS33B
• UniProt Synonym Gene Names: hVPS33B
• UniProt Entry Name: VP33B_HUMAN

NCBI Description

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

VPS33B: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Defects in VPS33B are the cause of arthrogryposis-renal dysfunction-cholestasis syndrome type 1 (ARCS1). ARCS1 is an autosomal recessive multisystem disorder, characterized by neurogenic arthrogryposis multiplex congenita, renal tubular dysfunction and neonatal cholestasis with bile duct hypoplasia and low gamma glutamyl transpeptidase activity. Platelet dysfunction is common. Belongs to the STXBP/unc-18/SEC1 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: clathrin-coated vesicle; cytoplasm; early endosome membrane; late endosome; late endosome membrane; lysosomal membrane; lysosome; perinuclear region of cytoplasm; recycling endosome

Molecular Function: protein binding

Biological Process: endosome organization and biogenesis; endosome to lysosome transport; lysosome localization; melanosome localization; protein transport; vesicle docking during exocytosis; vesicle-mediated transport

Disease: Arthrogryposis, Renal Dysfunction, And Cholestasis 1