Rabbit anti-Human Vitamin K-dependent protein C Polyclonal Antibody | anti-PROC antibody

Anti-Vitamin K-dependent protein C antibody

Cat. Num. AAA355196

• Gene Names: PROC; PC; APC; PROC1; THPH3; THPH4
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: Vitamin K-dependent protein C; Polyclonal Antibody; Anti-Vitamin K-dependent protein C antibody; Polyclonal Anti- Vitamin K-dependent protein C; anti-PROC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid

• Applicable Applications for anti-PROC antibody: Western Blot (WB)
• Application Notes: WB (1:2000)
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the region of human Vitamin K-dependent protein C.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles

Western Blot (WB)

Related Product Information for anti-PROC antibody

Vitamin K-dependent protein C, also called Protein C(PROC), is a vitamin K-dependent plasma glycoprotein. The PROC gene contains 9 exons and spans about 11 kb. [1] Protein C synthesis occurs in the liver, it is a major component in anticoagulation in the human body. Activated protein C(APC) has been studied as way of treating lung injury, also has been considered for use in improving patient outcome in cases of ischemic stroke, a medical emergency in which arterial blockage deprives a region of brain of oxygen, causing tissue death. [2]

References

1. Plutzky, J., Hoskins, J. A., Long, G. L., Crabtree, G. R. Evolution and organization of the human protein C gene. Proc. Nat. Acad. Sci. 83: 546-550, 1986. 2. Mosnier LO, Zlokovic BV, Griffin JH (April 2007). "The cytoprotective protein C pathway". Blood 109 (8): 3161-72.

NCBI and Uniprot Product Information

• NCBI GI #: 4506115
• NCBI GeneID: 5624
• NCBI Accession #: NP_000303.1
• NCBI GenBank Nucleotide #: NM_000312.3
• UniProt Accession #: P04070; Q15189; Q15190; Q16001; Q53S74; Q9UC55
• Molecular Weight: 52,071 Da
• NCBI Official Full Name: vitamin K-dependent protein C preproprotein
• NCBI Official Synonym Full Names: protein C (inactivator of coagulation factors Va and VIIIa)
• NCBI Official Symbol: PROC
• NCBI Official Synonym Symbols: PC; APC; PROC1; THPH3; THPH4
• NCBI Protein Information: vitamin K-dependent protein C; autoprothrombin IIA; anticoagulant protein C; blood coagulation factor XIV
• UniProt Protein Name: Vitamin K-dependent protein C
• Protein Family: Vitamin K-dependent protein
• UniProt Gene Name: PROC
• UniProt Entry Name: PROC_HUMAN

NCBI Description

This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009]

Uniprot Description

Function: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids.

Catalytic activity: Degradation of blood coagulation factors Va and VIIIa.

Subunit structure: Synthesized as a single chain precursor, which is cleaved into a light chain and a heavy chain held together by a disulfide bond. The enzyme is then activated by thrombin, which cleaves a tetradecapeptide from the amino end of the heavy chain; this reaction, which occurs at the surface of endothelial cells, is strongly promoted by thrombomodulin.

Tissue specificity: Plasma; synthesized in the liver.

Post-translational modification: The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans. Ref.12 Ref.15The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.

Involvement in disease: Thrombophilia due to protein C deficiency, autosomal dominant (THPH3) [MIM:176860]: A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. Individuals with decreased amounts of protein C are classically referred to as having type I protein C deficiency and those with normal amounts of a functionally defective protein as having type II deficiency.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8 Ref.19 Ref.20 Ref.21 Ref.22 Ref.25 Ref.26 Ref.30 Ref.31 Ref.32 Ref.36 Ref.39 Ref.40 Ref.41Thrombophilia due to protein C deficiency, autosomal recessive (THPH4) [MIM:612304]: A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. It results in a thrombotic condition that can manifest as a severe neonatal disorder or as a milder disorder with late-onset thrombophilia. The severe form leads to neonatal death through massive neonatal venous thrombosis. Often associated with ecchymotic skin lesions which can turn necrotic called purpura fulminans, this disorder is very rare.Note: The disease is caused by mutations affecting the gene represented in this entry.

Miscellaneous: Calcium also binds, with stronger affinity to another site, beyond the GLA domain. This GLA-independent binding site is necessary for the recognition of the thrombin-thrombomodulin complex.

Sequence similarities: Belongs to the peptidase S1 family.Contains 2 EGF-like domains.Contains 1 Gla (gamma-carboxy-glutamate) domain.Contains 1 peptidase S1 domain.

Sequence caution: The sequence S76088 differs from that shown. Reason: Erroneous termination at position 151. Translated as Cys.