Human Vitamin K-dependent protein C ELISA Kit | PROC elisa kit

Human Vitamin K-dependent protein C ELISA Kit

Cat. Num. AAA2884858

• Gene Names: PROC; PC; APC; PROC1; THPH3; THPH4
• Reactivity: Human
• Synonyms: Vitamin K-dependent protein C; Human Vitamin K-dependent protein C ELISA Kit; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; PROC; 3.4.21.69; PROC elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Vitamin K-dependent protein C. No significant cross-reactivity or interference was observed.
• Sequence Length: 461

• Assay Type: Sandwich
• Detection Range: 0.78-50 ng/mL
• Sensitivity: 0.195 ng/mL
• Intra-Assay CV: <=7.5%
• Inter-Assay CV: <=10.8%
• Recovery: 99%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PROC elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Vitamin K-dependent protein C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin K-dependent protein C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Vitamin K-dependent protein C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Vitamin K-dependent protein C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Vitamin K-dependent protein C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506115
• NCBI GeneID: 5624
• NCBI Accession #: NP_000303.1
• NCBI GenBank Nucleotide #: NM_000312.3
• UniProt Accession #: P04070; Q15189; Q15190; Q16001; Q53S74; Q9UC55; B4DPQ7
• Molecular Weight: 57,556 Da
• NCBI Official Full Name: vitamin K-dependent protein C preproprotein
• NCBI Official Synonym Full Names: protein C, inactivator of coagulation factors Va and VIIIa
• NCBI Official Symbol: PROC
• NCBI Official Synonym Symbols: PC; APC; PROC1; THPH3; THPH4
• NCBI Protein Information: vitamin K-dependent protein C
• UniProt Protein Name: Vitamin K-dependent protein C
• Protein Family: Vitamin K-dependent protein
• UniProt Gene Name: PROC
• UniProt Entry Name: PROC_HUMAN

NCBI Description

This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009]

Uniprot Description

PROC: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal dominant (THPH3). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Individuals with decreased amounts of protein C are classically referred to as having type I protein C deficiency and those with normal amounts of a functionally defective protein as having type II deficiency. Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal recessive (THPH4). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. It results in a thrombotic condition that can manifest as a severe neonatal disorder or as a milder disorder with late-onset thrombophilia. The severe form leads to neonatal death through massive neonatal venous thrombosis. Often associated with ecchymotic skin lesions which can turn necrotic called purpura fulminans, this disorder is very rare. Belongs to the peptidase S1 family.

Protein type: Protease; EC 3.4.21.69; Apoptosis

Chromosomal Location of Human Ortholog: 2q13-q14

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi apparatus; Golgi lumen

Molecular Function: calcium ion binding; protein binding; serine-type endopeptidase activity

Biological Process: blood coagulation; cellular protein metabolic process; ER to Golgi vesicle-mediated transport; leukocyte migration; negative regulation of apoptosis; negative regulation of blood coagulation; negative regulation of coagulation; negative regulation of inflammatory response; peptidyl-glutamic acid carboxylation; post-translational protein modification; proteolysis; signal peptide processing

Disease: Thrombophilia Due To Protein C Deficiency, Autosomal Dominant; Thrombophilia Due To Protein C Deficiency, Autosomal Recessive