Rabbit VISA Polyclonal Antibody | anti-VISA antibody

VISA Antibody

Cat. Num. AAA9413837

• Gene Names: MAVS; IPS1; VISA; IPS-1; CARDIF
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: VISA; Polyclonal Antibody; VISA Antibody; Virus-induced signaling adapter; mitochondrial antiviral signaling protein; MAVS; CARD adapter inducing interferon-beta; Cardif; IPS-1; anti-VISA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 540

• Applicable Applications for anti-VISA antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 13 amino acid peptide from near the amino terminus of human VISA.
• Target Name: VISA
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of VISA in A20 cell lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of VISA in human brain tissue with VISA antibody at 5 ug/mL.)

Related Product Information for anti-VISA antibody

Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN-b signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-kB. VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKKa, IKKb and IKKeta kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. At least three isoforms of VISA are known to exist.

Product Categories/Family for anti-VISA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 83776598
• NCBI GeneID: 57506
• NCBI Accession #: NP_065797
• NCBI GenBank Nucleotide #: NM_020746.4
• Molecular Weight: 15,012 Da
• NCBI Official Full Name: mitochondrial antiviral-signaling protein isoform 1
• NCBI Official Synonym Full Names: mitochondrial antiviral signaling protein
• NCBI Official Symbol: MAVS
• NCBI Official Synonym Symbols: IPS1; VISA; IPS-1; CARDIF
• NCBI Protein Information: mitochondrial antiviral-signaling protein
• UniProt Protein Name: Mitochondrial antiviral-signaling protein
• UniProt Gene Name: MAVS
• UniProt Synonym Gene Names: IPS1; KIAA1271; VISA; MAVS; Cardif; IPS-1; VISA
• UniProt Entry Name: MAVS_HUMAN

NCBI Description

This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral immunity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

MAVS: Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. Interacts with DDX58/RIG-I, IFIH1/MDA5, TRAF2 and TRAF6. May interact with IRF3, FADD, RIPK1, CHUK and IKBKB. Does not interact with TBK1. Interacts with and is cleaved by HCV and hepatitis GB virus B NS3/4A proteases. Interacts with and is cleaved by HHAV protein 3ABC. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS/IPS1, PCBP2 and ITCH. Interacts with CYLD. Interacts with SRC. Interacts with DHX58/LGP2 and IKBKE. Present in T-cells, monocytes, epithelial cells and hepatocytes. Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: peroxisomal membrane; mitochondrial outer membrane; mitochondrion; mitochondrial membrane; integral to membrane

Molecular Function: protein binding; signal transducer activity; CARD domain binding; protein kinase binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of protein import into nucleus, translocation; positive regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-8 production; negative regulation of viral genome replication; defense response to bacterium; positive regulation of transcription factor import into nucleus; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation; positive regulation of defense response to virus by host; activation of innate immune response; defense response to virus; negative regulation of interferon type I production; positive regulation of interferon-alpha production