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Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) Recombinant Protein | ENPP1 recombinant protein
Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1)
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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NCBI and Uniprot Product Information
Accurate Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions.
NCBI Description
mutation of the human homolog is detected in patients with idiopathic infantile arterial calcification [RGD, Feb 2006]
Uniprot Description
ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Protein type: Phosphatase (non-protein); Motility/polarity/chemotaxis; EC 3.1.4.1; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - riboflavin; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Nucleotide Metabolism - purine; Phosphodiesterase; EC 3.6.1.9
Cellular Component: extracellular space; cell surface; lysosomal membrane; basolateral plasma membrane; integral to plasma membrane; plasma membrane; extracellular region; integral to membrane
Molecular Function: phosphodiesterase I activity; nucleotide diphosphatase activity; protein homodimerization activity; nucleic acid binding; zinc ion binding; metal ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; ATP binding; insulin receptor binding; scavenger receptor activity; polysaccharide binding
Biological Process: receptor-mediated endocytosis; generation of precursor metabolites and energy; sequestering of triacylglycerol; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; bone remodeling; negative regulation of fat cell differentiation; phosphate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; immune response; negative regulation of protein amino acid autophosphorylation; negative regulation of cell growth; inorganic diphosphate transport; negative regulation of glycogen biosynthetic process
Research Articles on ENPP1
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Product Notes
The ENPP1 enpp1 (Catalog #AAA2011001) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) reacts with Rattus norvegicus (Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the ENPP1 enpp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ESH GSLNHLLKKP IYTPSHPKEE SFLSQCPIKS VSSDLGCTCD PSIVPIMDFE KQFNLTTDAV EDVYSMTVPN GRPRNLQKQH RVCLLHQQQF LTGYSLDLLM PLWTSYTFLS NDQFSTDDFS NCLYQDLRIP LSPMHKCSYY KSTSKLSYGF LTPPRLNRVS RQIYSEALLT SNIVPMYQSF QVIWQYLHDT VLRRYAQERN GVNVVSGPVF DFDYDGRYDS SEI. It is sometimes possible for the material contained within the vial of "Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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