Rabbit anti-Mouse VE Cadherin Polyclonal Antibody | anti-VE antibody

VE Cadherin Polyclonal Antibody

Cat. Num. AAA3013670

• Gene Names: Cdh5; 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: VE Cadherin; Polyclonal Antibody; VE Cadherin Polyclonal Antibody; VE-cadherin; Cdh5; 7B 4; 7B4; 7B4 antigen; CADH5; Cadherin 5; Cadherin 5 type 2; type 2 (vascular endothelium); type 2; VE cadherin (vascular epithelium); cadherin; vascular endothelial; 1; Cadherin-5; Cadherin5; CD 144; CD144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Endothelial specific cadherin; FLJ17376; OTTHUMP00000174777; Vascular endothelial cadherin; Vascular epithelium cadherin; VE Cad; VEC; anti-VE antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VE Cadherin polyclonal antibody detects endogenous levels of VE Cadherin protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 784

• Applicable Applications for anti-VE antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000-1:5,000
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of VE Cadherin on different lysates using anti-VE Cadherin antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Mouse placenta Lane 3: Mouse spleen Lane 4: Mouse lung)

Related Product Information for anti-VE antibody

The cadherins are a family of Ca²⁺-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy-terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. VE-cadherin (for vascular endothelial cadherin, also designated cadherin-5) is localized at intercellular junctions of endothelial cells, where it is thought to play a role in the cohesion and organization of intercellular junctions.

NCBI and Uniprot Product Information

• NCBI GI #: 38490561
• NCBI GeneID: 12562
• NCBI Accession #: NP_033998.2
• NCBI GenBank Nucleotide #: NP_033998.2
• UniProt Accession #: P55284
• Molecular Weight: 88/125kDa
• NCBI Official Full Name: cadherin-5 preproprotein
• NCBI Official Synonym Full Names: cadherin 5
• NCBI Official Symbol: Cdh5
• NCBI Official Synonym Symbols: 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
• NCBI Protein Information: cadherin-5
• UniProt Protein Name: Cadherin-5
• UniProt Gene Name: Cdh5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_MOUSE

NCBI Description

This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Mice lacking the encoded protein die in utero due to vascular insufficiency, caused by increased endothelial apoptosis. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8. [provided by RefSeq, Oct 2015]

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: adherens junction; cell junction; cell surface; external side of plasma membrane; intercellular junction; tight junction

Molecular Function: beta-catenin binding; protein binding; protein phosphatase binding; receptor binding

Biological Process: blood vessel maturation; cell-cell adhesion; intercellular junction assembly; negative regulation of cell proliferation; transforming growth factor beta receptor signaling pathway