VE-Cadherin Recombinant Protein | CDH5 recombinant protein

VE-Cadherin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122603

• Gene Names: Cdh5; 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
• Purity: >90% as determined by SDS-PAGE
• Synonyms: VE-Cadherin; VE-Cadherin Protein; Mouse; Recombinant (His Tag); Mouse VE-Cadherin/CD144/CDH5 Protein (His Tag); 7B4 Protein; AA408225 Protein; Cd144 Protein; VE-Cad Protein; Vec Protein; VEcad Protein; VECD Protein; cadherin 5; type 2 (vascular endothelium); CDH5 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala592

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 25 & Asp 46
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CDH5 (NP_033998.2) extracellular domain (Met 1-Ala 592) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDH5 recombinant protein

Background: Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based identify, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.

Product Categories/Family for CDH5 recombinant protein

Hemangioblast Markers Cardiac Stem Cell Markers; Canonical Wnt Pathway

References

Taveau JC, et al. (2008) Structure of artificial and natural VE-cadherin-based adherens junctions. Biochem Soc Trans. 36(Pt 2): 189-93.Vestweber D. (2008) VE-cadherin: the major endothelial adhesion molecule controlling cellular junctions and blood vessel formation. Arterioscler Thromb Vasc Biol. 28(2): 223-32.Gavard J. (2009) Breaking the VE-cadherin bonds. FEBS Lett. 583(1): 1-6.Vestweber D, et al. (2009) Cell adhesion dynamics at endothelial junctions: VE-cadherin as a major player. Trends Cell Biol. 19(1): 8-15.Boda-Heggemann J, et al. (2009) Beyond vessels: occurrence and regional clustering of vascular endothelial (VE-)cadherin-containing junctions in non-endothelial cells. Cell Tissue Res. 335(1): 49-65.

NCBI and Uniprot Product Information

• NCBI GI #: 38490561
• NCBI GeneID: 12562
• NCBI Accession #: NP_033998.2
• NCBI GenBank Nucleotide #: NM_009868.4
• UniProt Accession #: P55284; O35542
• Molecular Weight: 87,903 Da
• NCBI Official Full Name: cadherin-5 preproprotein
• NCBI Official Synonym Full Names: cadherin 5
• NCBI Official Symbol: Cdh5
• NCBI Official Synonym Symbols: 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
• NCBI Protein Information: cadherin-5
• UniProt Protein Name: Cadherin-5
• Protein Family: Cadherin
• UniProt Gene Name: Cdh5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_MOUSE

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: adherens junction; cell junction; cell surface; external side of plasma membrane; intercellular junction; tight junction

Molecular Function: beta-catenin binding; protein binding; protein phosphatase binding; receptor binding

Biological Process: blood vessel maturation; cell-cell adhesion; intercellular junction assembly; negative regulation of cell proliferation; transforming growth factor beta receptor signaling pathway