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Western Blot (WB) (PIGX Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PIGX antibody detected the PIGX protein (arrow).)

Rabbit anti-Human, Mouse PIGX Polyclonal Antibody | anti-PIGX antibody

PIGX, NT (PIGX, Phosphatidylinositol-glycan biosynthesis class X protein) (PE)

Gene Names
PIGX; PIG-X
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PIGX; Polyclonal Antibody; NT (PIGX; Phosphatidylinositol-glycan biosynthesis class X protein) (PE); Phosphatidylinositol-glycan biosynthesis class X protein; anti-PIGX antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PIGX antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PIGX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-103 amino acids from the N-terminal region of human PIGX.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PIGX Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PIGX antibody detected the PIGX protein (arrow).)

Western Blot (WB) (PIGX Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PIGX antibody detected the PIGX protein (arrow).)

Immunohistochemistry (IHC)

(PIGX antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PIGX antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PIGX antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PIGX antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-PIGX antibody
Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM (By similarity).
Product Categories/Family for anti-PIGX antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,974 Da
NCBI Official Full Name
phosphatidylinositol-glycan biosynthesis class X protein isoform 2
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis class X
NCBI Official Symbol
PIGX
NCBI Official Synonym Symbols
PIG-X
NCBI Protein Information
phosphatidylinositol-glycan biosynthesis class X protein
UniProt Protein Name
Phosphatidylinositol-glycan biosynthesis class X protein
UniProt Gene Name
PIGX
UniProt Synonym Gene Names
PIG-X
UniProt Entry Name
PIGX_HUMAN

NCBI Description

This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

PIGX: Essential component of glycosylphosphatidylinositol- mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM. Belongs to the PIGX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Biological Process: cellular protein metabolic process; preassembly of GPI anchor in ER membrane; C-terminal protein lipidation; post-translational protein modification

Research Articles on PIGX

Similar Products

Product Notes

The PIGX pigx (Catalog #AAA6333936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIGX, NT (PIGX, Phosphatidylinositol-glycan biosynthesis class X protein) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PIGX can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PIGX pigx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIGX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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