Rabbit anti-Human, Mouse VAMP1 Polyclonal Antibody | anti-VAMP1 antibody

VAMP1 Rabbit pAb

Cat. Num. AAA4754930

• Gene Names: VAMP1; SYB1; SPAX1; VAMP-1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: VAMP1; Polyclonal Antibody; VAMP1 Rabbit pAb; SYB1; SPAX1; VAMP-1; anti-VAMP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-VAMP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Synthetic peptide of human VAMP1
• Conjugation: Unconjugated
• Molecular Function: Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined.
• Modification: Unmodified
• Cell Position: Cell junction, Cytoplasmic vesicle, Membrane, Mitochondrion, Mitochondrion outer membrane, Synapse, Synaptosome
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: 162473(VAMP1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 162473(VAMP1 Antibody) at dilution 1/35. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 162473(VAMP1 Antibody) at dilution 1/35. (Original magnification: ×200))

Product Categories/Family for anti-VAMP1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 7657673
• NCBI GeneID: 6843
• NCBI Accession #: NP_055046
• NCBI GenBank Nucleotide #: NM_014231.4
• UniProt Accession #: P23763; O75468; Q15857; Q6FG94; Q8IVC9; A8MVP3; D3DUR3
• Molecular Weight: 11.9 kDa (111 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: vesicle-associated membrane protein 1 isoform 1
• NCBI Official Synonym Full Names: vesicle associated membrane protein 1
• NCBI Official Symbol: VAMP1
• NCBI Official Synonym Symbols: SYB1; SPAX1; VAMP-1
• NCBI Protein Information: vesicle-associated membrane protein 1
• UniProt Protein Name: Vesicle-associated membrane protein 1
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP1
• UniProt Synonym Gene Names: SYB1; VAMP-1
• UniProt Entry Name: VAMP1_HUMAN

NCBI Description

Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2014]

Uniprot Description

VAMP1: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p

Cellular Component: SNARE complex; synaptic vesicle; mitochondrial outer membrane; neuron projection; cell surface; synaptic vesicle membrane; integral to plasma membrane; cell junction; cytosol

Molecular Function: SNAP receptor activity; SNARE binding; protein binding

Biological Process: vesicle fusion; exocytosis; pathogenesis

Disease: Spastic Ataxia 1, Autosomal Dominant