Rabbit IRS1 / IRS-1 Polyclonal Antibody | anti-IRS1 antibody

Rabbit Polyclonal (IgG) to Human IRS1 / IRS-1

Cat. Num. AAA247122

• Gene Names: IRS1; HIRS-1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: IRS1 / IRS-1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human IRS1 / IRS-1; Anti-IRS1 / IRS-1 Antibody (aa289-338) IHC-plus; IRS1; IRS-1; HIRS-1; Insulin receptor substrate 1; Human IRS1; anti-IRS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IRS1 Antibody detects endogenous levels of total IRS1 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1242

• Applicable Applications for anti-IRS1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: ELISA (1:1000), IF (1:100 - 1:500), IHC-P (10 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human IRS-1 (aa289-338).
• Immunogen: IRS1 / IRS-1 antibody was raised against synthetic peptide from human IRS-1 (aa289-338).
• Antigen Modification: aa289-338
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-IRS1 antibody IHC of human intestine, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-IRS1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of COS7 cells, using IRS-1 (Ab-323) Antibody. The picture on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 5031805
• NCBI GeneID: 3667
• NCBI Accession #: NP_005535.1
• NCBI GenBank Nucleotide #: NM_005544.2
• UniProt Accession #: P35568
• Molecular Weight: 131,591 Da
• NCBI Official Full Name: insulin receptor substrate 1
• NCBI Official Synonym Full Names: insulin receptor substrate 1
• NCBI Official Symbol: IRS1
• NCBI Official Synonym Symbols: HIRS-1
• NCBI Protein Information: insulin receptor substrate 1; IRS-1
• UniProt Protein Name: Insulin receptor substrate 1
• Protein Family: Isoleucine--tRNA ligase
• UniProt Gene Name: IRS1
• UniProt Synonym Gene Names: IRS-1
• UniProt Entry Name: IRS1_HUMAN

NCBI Description

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Uniprot Description

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; nucleus; cytosol

Molecular Function: insulin-like growth factor receptor binding; signal transducer activity; protein binding; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; signal transduction; glucose homeostasis; response to insulin stimulus; negative regulation of insulin secretion; positive regulation of glucose import; mammary gland development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent